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Articles by Saad A. Alamri
Total Records ( 4 ) for Saad A. Alamri
  Hani A. Alhadrami , Ken S. Killham and Saad A. Alamri
  A novel flow bioreactor design was used in this study to minimise the toxic effect of leachate obtained from a landfill site. The bioreactor used comprised sand inoculated with microbial hydrocarbon degraders to degrade the various contaminants in the landfill leachate. Prior to bioreactor testing, optimal leachate test concentrations were identified as 10 and 20% by toxicity screening with a lux bacterial biosensor. The bioreactor performance was assessed by carrying out different types of physical, chemical and biological measurements. In conclusion, the bioreactor designed in this study used was effective in treating the leachate in terms of hydrocarbon degradation. Conductivity and pH showed to have no significant effect on the bioreactor performance. This may indicate that the effect of some environmental factors might be site dependent.
  Saad A. Alamri
  Microflora including bacteria, actinomycetes, yeasts and filamentous fungi recovered from the leaves of Juniperus procera collected from two different altitudes at January and July 2007 from Aseer region, Saudi Arabia. Types and numbers of microflora varied according to the altitude and the month of collection. The number of microflora was higher on old leaves than young ones in most cases. Low altitude exhibited more microflora than high altitude. The relationship between meteorological factors and type and number of the recovered microflora was investigated. Inoculation of detached healthy leaves of Juniperus procera by predominant fungal isolates revealed that only Alternaria alternata as a pathogen of this plant. To confirm the pathogenicity of this fungus, scanning and transmission electron microscopic examination revealed the colonization of this pathogen inside the leaf tissue. Penetration of Juniperus leaves by the fungus occurred only through stomata and the invading hyphae were located in the intercellular spaces of leaf tissues. Bacteria also observed inside the intercellular spaces of leaf tissues of the host plant and not inside the leaf cells. Adjacent host cells to bacteria were also affected. Ultrastructural changes in the infected cells, from inoculated leaves, included changes in chloroplasts, nuclei and mitochondria.
  Saad A. Alamri
  A suite of microbiological tools, complementing the standard chemical analysis, was used for evaluating the effect of steam and surfactant on the rate of hydrocarbon biodegradation. The microbiological monitoring tools were soil microbial counts, soil microbial respiration, and microbial toxicity biosensor. The correlations between these parameters and with the levels of hydrocarbon residues were investigated. The overall assessment showed that bioremediation was an effective method for reducing hydrocarbon concentration. However, the monitoring tools used showed that the steam and surfactant had no significant effect in increasing the rate of hydrocarbon bioremediation or the toxicity reduction comparing with the control. Consequently, alternative techniques for enhancing hydrocarbon bioavailability must be investigated in order to establish a successful bioremediation of heavy hydrocarbons in soil. This study also demonstrated that the combination of different classes of biological and chemical tools would be more effective in monitoring hydrocarbon bioremediation that any single approach.
  Saad A. Alamri , Mohamed Hashem , Sulaiman A. Alrumman and Malak S.A. Al- Qahtani
  This study aimed to enhance the bio-ethanol production from date molasses as a cheap and renewable resource by local species of non-Saccharomyces yeasts by optimization the production’s conditions. Hanseniaspora guilliermondii KKUY-0036 and H. uvarum KKUY-0078 were used based on their ability to ferment the date molasses efficiently. They were identified by the sequencing of D1/D2 domain of the 26S rRNA gene. Their identity was confirmed by comparing the obtained sequence with similar sequences allocated in the GenBank. To enhance the ethanol productivity, temperature, pH, fermentation period, molasses concentration and addition of some elements were optimized. Results revealed that the two yeast species exhibited their maximum productivity of ethanol at 30°C when they were grown on 20-25% of the date molasses after 96-120 h of incubation. The highest ethanol concentration was achieved in weak acidic medium (pH 4-6). Addition of zinc, magnesium and manganese induced the production of ethanol by the two yeasts and the optimum concentrations were 0.6, 0.2-0.3 and 0.03 g L-1, respectively. The study introduces both H. guilliermondii KKUY-0036 and H. uvarum KKUY-0078 as new ethanol-producers that ferment date molasses efficiently and will greatly reduce the biofuel production cost.
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