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Fifteen isolates of Pasteurella multocida from clinical cases of haemorrhagic septicemia in cattle and buffaloes were confirmed by morphological, cultural, biochemical characteristics and slide agglutination test using antiserum against Robert type-I. Enrofloxacin and norfloxacin were highly sensitive to 93.7 and 75 percent of isolates, respectively and no resistance was observed, followed by gentamicin (62.5%), amoxacillin (37.5%), combination of trimethoprim+sulphamethoxazole (31.2%) and ampicillin (18.7%). Norfloxacin and enrofoxacin should be considered as drug of choice in haemorrhagic septicemia keeping in view acute nature of the disease. Use of ampicillin and amoxacillin should be discouraged due to the emergence of β lactamase activity in isolates. The maximum percentage of isolates was resistant to erythromycin (81.2%) and kanamycin (62.5%), followed by oxytetracycline (37.5%) and doxycycline (25%).
