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Articles by S.R. Gulhane
Total Records ( 4 ) for S.R. Gulhane
  D.H. Tambekar , D.V. Dhanorkar , S.R. Gulhane and M.N. Dudhane
  In the present study, attempt was made to isolate bacterial pathogens in blood and determination of their antibiogram. A total of 448 blood samples were analyzed. Out of which 405 (92%) samples of aerobic blood culture and 43 (8%) samples of anaerobic blood culture. From aerobic culture 111 (27%) pathogens and from anaerobic culture 6 (13%) pathogens were identified. In aerobic culture Staphylococcus aureus 71 (64.54%) was the most common cause of bacteraemia followed by Listeria monocytogenes 17 (15.45%), Diplococcus sp. 7 (6.36%), Salmonella typhi 6 (5.45%), Streptococci sp. 4 (3.63%), Klebsiella pneumoniae 3 (2.72%) and Campylobacter sp., E. coli and Haemophilus influenzae (single isolates of each, respectively). From anaerobic culture Clostridium perfringens 6 (13%) were identified. The antibiotic sensitivity test was done by disc diffusion method. The antibiogram of these pathogens showed resistance to cefotaxime, cefadroxil, cefaclor, cefuroxime, ceftazidime, ceftriazone and sensitivity to meropenem, linezolid and amikacin. Thus study indicated that the bacterial blood pathogens are becoming resistant to commonly used antibiotics, which may be due to indiscriminate use of these antibiotics. So it is very much important to have culture and sensitivity test of concern pathogens.
  D.H. Tambekar , S.R. Gulhane and Y.S. Banginwar
  A comprehensive bacteriological analysis of 525 drinking water samples collected from railway stations, houses, rivers and lakes (surface water), tube wells and open wells was carried out using Manja`s H2S field test, MTFT (MPN) test, TTC (Eijkman test) and MFT test. Detection efficiency of faecal coliform contamination for H2S field test (96-97%) was comparable to MPN test and more detection level over that of MFT and TTC. Efficiency of H2S test varies with the source and decreased with the depth of the source of water. However, the H2S test, compared to other tests, was more suitable, reliable, inexpensive, easy to perform and useful to detect fecal contamination in drinking water within 24 h, for places where time, man and laboratory facilities are very poor.
  D.H. Tambekar , P.B. Gulhane , K.S. Goyal and S.R. Gulhane
  The quality of dental unit water is of considerable importance since patients and dental staff are regularly exposed to water and aerosols generated from the dental unit waterlines (DUWLs). Pseudomonas aeruginosa contamination in dental unit waterlines was reported by its origin from incoming local water supplies of the dental clinics. A total of 82 dental unit water samples from 34 dental clinics of Amravati city were collected, out of which 59 water samples were contaminated by P. aeruginosa. The ultrasonic scaler showed maximum 81% contamination of P. aeruginosa then other two hand-pieces, 3 in 1 syringe 68% and air rotor 69%. The dental clinics using distilled water as a source of water for treatment showed 72% P. aeruginosa contamination whereas Undergraduate (UG) dentist’s clinics showed 75% and Postgraduates (PG) dentist’s clinics showed 68% P. aeruginosa in DUWLs water.
  D.H. Tambekar , N.B. Hirulkar , D.D. Bhokre , S.R. Gulhane and Y.S. Banginwar
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