Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
 
Articles by S.M.A. Kawsar
Total Records ( 4 ) for S.M.A. Kawsar
  S.M.A. Kawsar , E. Huq , N. Nahar and Y. Ozeki
  Extracts of Macrotyloma uniflorum plants were examined as potential sources of phenolic compounds. Reversed phase high performance liquid chromatography (RP-HPLC) with UV detection was employed for the identification and quantification of the phenolic acids. Eight phenolic acids, namely, 3, 4-dihydroxy benzoic, p-hydroxy benzoic, vanillic, caffeic, p-coumeric, ferulic, syringic and sinapic acids were isolated from an ethanolic extract of Macrotyloma uniflorum. The most abundant phenolic acids were p-coumaric acid (8.95 mg 10-2 g of dry sample) and p-hydroxy benzoic acid (7.81 mg/100 g of dry sample).
  S.M.A. Kawsar , G. Mostafa , E. Huq , N. Nahar and Y. Ozeki
  The bioactivity guided separation of the dichloromethane extract of the aerial parts of Macrotyloma uniflorum Linn. resulted in the isolation of methyl ester of hexadecanoic and ethyl ester of hexadecanoic acid mixture (I) and n-hexadecanoic acid (II). The structures of the isolated compounds were elucidated by spectroscopic analysis, including UV, IR, 1H-NMR, 13C-NMR and mass spectroscopy. In addition, the fractionated crude extract of 1-butanol exhibited the significant hemolytic activity by using mouse erythrocytes.
  S.M.A. Kawsar , E. Huq and N. Nahar
  The fractionated crude extracts dichloromethane (CH2Cl2), ethyl acetate (EtOAc), 1-butanol (1-BuOH) and aqueous (H2O) from aerial parts of Macrotyloma uniflorum were screened for cytotoxicity using the brine shrimp lethality bioassay technique. Most of the extracts were found to be non-toxic and this indicates that the ethnobotanical use (oral applications) of the experimental plant are justified.
  Y. Fujii , S.M.A. Kawsar , R. Matsumoto , H. Yasumitsu , N. Kojima and Y. Ozeki
  To find novel carbohydrate-binding proteins (lectins) from marine invertebrates to understand the binding mechanism of the protein and to apply it for glycan-dependent diagnostics and/or glycoconjugates capture technology. A D-galactoside-binding lectin was purified from foot of bladder moon shell, Glossaulax didyma by lactosyl-agarose affinity chromatography. The crude supernatant by Tris-buffered saline had strong hemagglutination activity against trypsinized and glutaraldehyde-fixed human erythrocyte. However, the activity was not inhibited by any tested saccharides and chilete reagents. On the other hand, the dialyzed crude supernatant obtained from the precipitates with 100 mM lactose in Tris-buffered saline had also hemagglutination activity inhibited by β-galactoside and D-galactose. The lectin was purified with lactosyl-agarose affinity chromatography. The molecular mass of the lectin was determined to be 60 kDa by SDS-PAGE under reducing and non-reducing conditions and being a 60 kDa polypeptide monomer by gel permeation chromatography. The association-rate constant (kass) and dissociation-rate constant (kdiss) determined for the lectin against asialofetuin was determined as 5.4x104 M-1sec-1 and 7.2x10-3sec-1, respectively. Lectin-conjugated Sepharose gel captured asialofetuin and eluted it by lactose-containing buffer from the gel, indicating that the lectin could catch the asialoglycoprotein. It was concluded that a many amount of a D-galactoside-binding lectin which can catch asialoglycoprotein presents in foot of the bladder moon shell.
 
 
 
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility