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Articles by S.M. Nurul Amin
Total Records ( 3 ) for S.M. Nurul Amin
  Nur Syuhada Mat Noor , Aziz Arshad , S.M. Nurul Amin and Mohd Salleh Kamarudin
  Background and Objective: Copepods are being used widely as live feed in aquaculture industry particularly for larval rearing of fish and crustacean. Several marine copepods have been cultured successfully before but the numbers of species documented is still limited. Cyclopoid copepod, Oithona simplex is readily available in the Malaysian coastal waters and their high abundance is observed in the coastal waters of Port Dickson, Malaysia. It is targeted as a potential live food for larval rearing of marine fish species. The present study was conducted to determine the effect of environmental parameters viz. salinity, temperature, light intensity and photoperiod on the reproductive cycle of cyclopoid copepod, Oithona simplex under laboratory condition. Materials and Methods: Four different salinity levels (20, 25, 30 and 35 ppt), three different temperatures set up (25, 30 and 35°C), three light intensities expose (5, 20, 35 μmol m2 sec1) and three different photoperiod phases (12 h light (L):12 h dark (D), 1 h (L):23 h (D), 24 h (L):0 h (D) were adopted during this experiment. Results were subjected to one-way ANOVA. Results: The results showed that the optimum salinity was recorded at 30 ppt for the highest production of off springs. Similar highest production was found at 35°C temperature setup. The optimum light intensity expose was under 5 μmol m2 sec1 and this was reflected by the increase in reproductive activity and decreased in development period. Subsequently, 12 h light:12 h dark treatment resulted in the highest reproduction process. Conclusion: It could be recommended that optimal environmental condition which comprised of 30 ppt salinity, 35°C temperature, 5 μmol m2 sec1 light intensity and alternate 12 h light and dark photoperiod is required for culturing of O. simplex in captivity. This is the first attempt to breed and mass production in controlled environment. The information gathered from the experiment could be helpful to produce a mass culture of O. simplex indoor for the purpose of live food supply in the hatchery.
  S.Y. Oh , A. Arshad , S.P. Pang and S.M. Nurul Amin
  Catch composition of Estuarine Set Bag Net (ESBN) in coastal waters of Pontian, Johor, Peninsular Malaysia was investigated based on the catch data during June to November 2007. The total catches comprised of three major groups were Acetes shrimps (89%), juvenile fishes (9%) and other shrimps (2%). Among the Acetes shrimps, three species were recorded from the study area viz., Acetes indicus (74%), Acetes serrulatus (24%) and Acetes japonicus (2%). The nine juvenile fishes species were identified as Setipinna brevicep, Trichiurus lepturus (ribbon fish), Lutjanus malabaricus, Epinephelus diacanthus, Cynoglossus bilineatus, Secutor ruconius, Lactarius lactarius, Atelopus japonicus and Ilisha kampeni. Other shrimps were constituted of two families as Penaeidae (70%) and Thalassocaridae (30%). This study revealed that there is no bad impact of ESBN on juvenile fishes.
  D. Aziz , S.S. Siraj , A. Arshad , S.M. Nurul Amin and S.A. Harmin
  Genomic DNA of sergestid shrimp, Acetes japonicus was successfully extracted by using the Promega Wizard Genomic DNA Purification Kit. Population genetic characterization of A. japonicus along the western coast of Peninsular Malaysia (state of Perak, Malacca and Kedah) was examined using the Random Amplified Polymorphic DNA (RAPD) marker. Twenty oligonucleotides from operon A kit were used to screen the populations, of which 6 were able to be amplified (OPA03, OPA04, OPA07, OPA09, OPA10, OPA16). The percentages of polymorphic bands of the three populations investigated varied from 57.77 to 87.77%. Genetic distances between populations and cluster analysis from UPGMA grouped the populations into two major clusters. The Perak and Malacca populations were in one cluster, while the Kedah population was clustered by itself, indicating a different population. The genetic distance was highest as 0.0999 for the Kedah and the Malacca populations while lowest value was 0.0413 for the Perak and the Malacca populations, which probably have a closed ancestral relationship. The results of this study suggested that RAPD analysis, if carried out carefully and accurately would give a good indication of the separation between individuals of different populations and is suitable for identification of closely related genotypes.
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