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Articles by S.L. Wu
Total Records ( 3 ) for S.L. Wu
  G.J. Dai , J.Y. Wang , O. Olajide , Q.S. Li , H. Shen , K.Z. Xie , Z.Y. Wang , S.L. Wu , Y.P. Gu and G.X. Zhang
  In order to develop rapid and reliable SCAR markers linked to the QTL, to get better understood of orgin of New Yangzhou chickens, two bands of OPAY02 type marker have been cloned, sequenced and two pairs of primer were designed according to the DNA sequence of two bands of OPAY02 to amplify the SCAR-markers on 86 individuals randomly selected from New Yangzhou chickens. The results of comparing the two bands DNA sequence with red jungle fowl genomic DNA database showed that the large molecular size band is located on the 3rd chromosome, the sequence identities were 98% and 8 SNPs mutations were detected, they were at 195 (T→G), 316 (A→T), 538 (G→ A), 731 (T→A), 1147 (G→A), 1329 (T→C), 1927 (C→ T) and 2081 (C→T). On the other hand, the small molecular size band can`t be found in red jungle chicken genomic DNA database, speculated that the source of New Yangzhou Chickens may originate from not only red jungle fowl, but also other jungle fowls. SCAR -markers confirmed that the two bands of OPAY02 type marker can be applied to genetic analyses because of its stability and reliability. Genotype equilibrium test showed that New Yangzhou chickens are in equilibrium on the two bands loci, two bands of OPAY02 type marker selection will be in favor of New Yangzhou chickens body weight breeding.
  B.C. Li , G.H. Chen , J. Qin , X.S. Wu , S.L. Wu and Z.T. Cai
  The aim of this study was to evaluate the efficiency of isolation and culture of PGCs from various tissues of chicken embryos at specific developmental stages including: the circulating blood of stage 14 embryos (hatched for 48-52hrs), the genital ridge of stage 19 embryos (hatched for 68-72hrs) and the gonad of stage 28 embryos (hatched for 128-132hrs). Ficoll density-gradient centrifugation is a standard method for the purification of PGCs from fetal blood. The genital ridge and gonadal tissue contain more PGCs in total but must first be digested with trypsin-EDTA to give a single cell suspension containing a mixture of PGCs and other contaminating cell types. In these experiments, we cultured PGCs from the genital ridge and from gonadal tissue before and after Ficoll density-gradient purification. In all cases, PGCs were subsequently cultured in TCM-199 medium supplemented with 10% fetal calf serum. The results demonstrated that trypsin-EDTA alone of the genital ridge of stage 19 embryos yielded a total of2.7 x 104 per embryo of which 89.5% were viable. After Ficoll density-gradient purification of these cells the yield was 1.8 x 104 of which 87.5% were viable. Processing of the gonadal tissue of stage 28 embryos yielded a total of 3.1 x 104 PGCs per embryo of which 90.0% were viable. It was clear that the PGC yield with trypsin-EDTA alone was higher (P< 0.01) than the yield of the full procedure which included the Ficoll density-gradient purification step. The results of PGC culture from the three developmental stages indicated that the survival time was longest (80-88 hours) for PGCs obtained from stage 19 embryos. At this stage, a large number of PGCs had accumulated in the genital ridge which facilitated the isolation procedure. These results suggest that the highest yield of PGCs per embryo can be achieved by trypsin-EDTA treatment of genital ridge tissue from stage 19 chicken embryos.
  H.H. Musa , S.L. Wu , C.H. Zhu , H.I. Seri and G.Q. Zhu
  Probiotics are defined as microbial food supplements which beneficially affect the host animal by improving its intestinal microbial balance. The probiotics were improved feed conversion for the target species, reduced morbidity or mortality and benefits for the consumer through improved product quality. In this study, we found that a combination of probiotics with different mechanisms of action could amplify the protective range of bio-therapeutic preparations and the potentiated probiotics are more effective than their components separately. Bacterial probiotics were effective in chickens, pigs and pre-ruminant calves, whereas yeasts and fungal probiotics were given better results in adult ruminants. Probiotics were enhanced the growth of many domestic animals improved the efficacy of forage digestion and quantity and quality of milk, meat and egg. Probiotics protected animals against pathogens, enhanced immune response, reduced antibiotic use and shows high index of safety. The trend for future could be focus on basic research to identify and characterize existing probiotics strains, determine optimal doses needed for certain strain and asses their stability through processing and digestion.
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