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Articles by S.L. Gupta
Total Records ( 3 ) for S.L. Gupta
  N. Jindal , N.K. Mahajan , D. Mittal , S.L. Gupta and R.S. Khokhar
  During the period from July, 1994 to June, 2003, the epidemiological data related to infectious bursal disease outbreaks in 795 broiler flocks was analyzed. The disease affected 8.89% flocks during the nine year period with morbidity, cumulative mortality and case fatality rate of 5.9, 3.63 and 61.43%, respectively. Most of the affected birds were dull, depressed and had ruffled feathers and yellowish white diarrhoea. Post mortem changes were mainly recorded in the bursa of Fabricius followed by changes in thigh and breast muscles, and in some cases in gizzard. The outbreaks were recorded more in winter season (391) than in summer (249) and rainy (155) seasons. Of the total outbreaks, 83.39% were recorded in broiler chicks of age 21-40 days while rest were in chicks of age less than 20 days or more than 40 days. However, per cent morbidity, cumulative mortality and case fatality rate were significantly more in chicks of age more than 50 days. The disease was recorded both in the vaccinated (472) as well as unvaccinated (323) flocks. However, per cent morbidity and cumulative mortality were comparatively more in the unvaccinated flocks as compared to the vaccinated ones. Improper vaccinations and existence of very virulent strains of IBD virus could be the reasons for outbreaks of disease even in the vaccinated flocks. Poor reporting system and lack of diagnostic facilities are the major constraints in knowing the exact status of the disease and the economic losses.
  D. Mittal , N. Jindal , S.L. Gupta , R.S. Kataria and A.K. Tiwari
  During the period from July 2002 to June 2003, infectious bursal disease (IBD) was suspected in 101 commercial flocks of broiler chickens on the basis of clinical and post mortem findings in some districts of the Haryana state, India. Bursal samples were collected randomly from 20 flocks for the detection of infectious bursal disease virus (IBDV) by reverse transcription- polymerase chain reaction (RT-PCR) and nested PCR assay. IBDV could be detected in 17 samples as evidenced by amplification of 643 bp fragment of the very variable region of VP2 gene of virus by agarose gel electrophoresis. The authenticity of the amplicons was further confirmed by nested PCR generating amplicons of 552 bp using internal primers. The results of the present study indicated that RT-PCR followed by nested PCR can be used to diagnose field outbreaks of IBD in poultry because of its rapidity, accuracy and sensitivity.
  K. Singh , N. Jindal , S.L. Gupta , A.K. Gupta and D. Mittal
  A total of 30 field samples (27 tissue samples comprising of either trachea, lungs or brain; and 3 allantoic fluid samples) were collected from broiler chickens for detection of Newcastle disease virus (NDV) by RT-PCR. In addition, vaccine strains (F, LaSota, R2B) were taken as positive control and trachea from unvaccinated healthy bird as negative control. Newcastle disease virus (NDV) was detected in five field samples as well as in all the vaccine strains by RT-PCR. All these samples as well as vaccine strains yielded a band of 356 bp on amplification of F region of NDV. Three more field samples yielded a band of 216 bp with nested PCR, thus making total eight field samples positive for NDV. The results of the present study indicated that RT-PCR followed by nested PCR can be used to detect NDV directly from tissue samples in poultry.
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