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by
S.J. Li |
Total Records (
2 ) for
S.J. Li |
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P. Gong
,
Y.Z. Gong
,
Y.P. Yang
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Y. Yang
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Y.P. Feng
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S.J. Li
and
X.L. Peng
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Sex in birds is determined genetically as in mammals and a Z-linked gene DMRT1 is required for testis development. But it remains possible that the W chromosome contains a similarly critical factor for female development. The W-linked gene PKCIW was put forward as the seductive candidate for avian sex determination and/or sex differentiation. Mis-expression and over-expression of PKCIW were performed respectively in male and female chicken embryo fibroblast with tet-on inducible expression system in this study. The results of RT-PCR showed that with the PKCIW recombinant vector introducing, PKCIW mRNA expression was significantly improved in female chicken embryo fibroblast and was close to that of the female controls and in male chicken embryo fibroblast, in which normally there was no expression. Both mis- and over-expression of PKCIW led to up-regulated expression of P450arom and down-regulated expression of AMH, while large increase of P450arom in female and large decrease of AMH in male appeared. With mis- and over-expression of PKCIW, there was no significant change of FOXL2, SOX9, DMRT1 and ATP5A1W mRNA expression. These results showed the possible regulatory effect of PKCIW on AMH and P450arom in transcription level. |
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X.D. Hu
,
K. Jia
,
G.H. Zhang
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Z.X. Lin
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H.Y. Xia
,
D.W. Zhang
,
X.B. Zeng
,
H.S. Qiao
,
H. Zhang
and
S.J. Li
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Several Enzyme-Linked Immunosorbent Assay (ELISA) methods exist to diagnose Bovine Tuberculosis (BTB). In this study, an ELISA method was established with a mixture of esat-6 and cfp-10 as the diagnostic antigens. ELISA tests on 160 bovine serum samples (80 from cattle with BTB; 80 from healthy cattle) demonstrated that the sensitivity was 76.3% (61/80) and the specificity was 97.5% (78/80). A further screening test using ELISA and PPD was conducted with 335 serum samples collected at random from eight dairy herds (5 problematic herds and 3 BTB-negative herds) in Guangdong, China. In the problematic herds, the positive ELISA rate was 7.9% (16/202) for anti-esat-6 and cfp-10 antibodies and 5.4% (11/202) in the PPD. This study demonstrated that ELISA with the use of the esat-6 and cfp-10 mixture of antigens is simple and sensitive and can be used to analyze large numbers of samples for the serodiagnosis of BTB. |
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