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Articles by S.H.Z. Ariffin
Total Records ( 2 ) for S.H.Z. Ariffin
  A.A.A. Hamid , S. Hamdan , S.H.Z. Ariffin and F. Huyop
  A phylogenetic analysis of an unknown strain AZZ2 isolated from volcanic area Gunung Sibayak Indonesia was performed. Their phylogenetic relationships were analysed using MEGA4 software® to ascertain its evolutionary distance by reconstructing a phylogenetic tree of these organisms. The evolutionary history and bootstrap consensus tree were inferred using the Neighbor-Joining method from 500 replicates. The tree is drawn to scale, with branch lengths (next to the branches) in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the p-distance method and were in the units of the number of base substitutions per site. Based on the partial 16S rDNA sequence determination, the strain showed high sequence similarity to Citrobacter sp. strain JC73/SL7. AZZ2 gene was also compared among known dehalogenase producing bacteria 16S rDNA genes. The results suggested that AZZ2 was closely related to the Serratia marcescens HL1. On the basis of phylogenetic identification only, AZZ2 was subjected to grow on 2,2-dichloropropionate (2,2DCP). The results suggested that strain AZZ2 can degrade 2,2DCP as expected similar to the characteristic of strain HL1 that can grow on halogenated compound. From this study, there was a possibility to predict the phenotype of newly isolated bacteria. The present findings also show that the evolutionary relationships of 16S rDNA gene strain AZZ2 were illustrated by phylograms and both topology are not in good agreement and may suggest an uncertainty of the origin of dehalogenases in volcanic area.
  Z. Yamamoto , I.M. Jaafar , R.M.A. Wahab , Z.Z. Ariffin , I.Z.Z. Abidin , S. Senafi and S.H.Z. Ariffin
  Orthodontic treatment may cause dental root resorption. The risk of dental root resorption increases in orthodontic treated-traumatised teeth. An approach to monitor root resorption is by monitoring inflammatory markers. Therefore, we observed the profiles of inflammatory markers namely Creatine Kinase (CK), nitric oxide (NO), lactate dehydrogenase (LDH) and aspartate aminotransferase (AST) to determine their importance in orthodontic treatment especially in traumatised and nontraumatised incisors. A total of 13 subjects (7 female, 6 male) participated in this study. Samples were collected from gingival crevicular fluid at baseline (M0), 2 weeks after 0.014 NiTi archwire (M1) and 2 weeks after 0.018 NiTi archwire (M2). All enzyme activities were measured spectrophotometrically at 340 nm. Griess assay was used to measure nitric oxide level. The NO concentration and activities of CK, LDH and AST were not significantly different between traumatised and nontraumatised incisors groups during observation period (p>0.05). Compared to M1, NO concentration decreased significantly in M2. Other comparisons of all four markers over period of archwire changes in both traumatised and nontraumatised incisors groups showed no significant differences. Inflammatory markers showed different activities or level as responses to previous archwire usage and archwire changes. All markers are not sensitive enough to be categorized as inflammatory markers during orthodontic treatment with two archwire changes.
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