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Articles by S.H. Chen
Total Records ( 6 ) for S.H. Chen
  L. Ai , S.H. Chen , Y.N. Zhang , X.N. Zhou , H. Li , M.X. Chen , J. Guo , Y.C. Cai , X.Q. Zhu and J.X. Chen
  The present study examined sequence variations in the Internal Transcribed Spacers (ITS) of nuclear ribosomal DNA (rDNA) and two mitochondrial DNA (mtDNA) regions, namely cytochromec oxidase subunit 1 (cox1), NADH dehydrogenase subunits 1 (nad1), among Metorchis orientalis metacercaria isolates from Guangxi in China. The sequences of ITS, pcox1 and pnad1 were amplified from 6 individual M. orientalis metacercariae and sequenced. The relevant sequences of other 7 trematode species belonging to 6 genera in 4 families were downloaded from GenBank and their phylogenetic relationships were re-constructed using the combined pcox1 and pnad1 mt DNA sequences with Trichinella spiralis as outgroup. The results showed that sequences of ITS rDNA, pcox1 and pnad1 of M. orientalis were 1131, 654 and 650 bp, respectively and they were quite conserved among the M. orientalis isolates. However, they were quite different from that of other species, phylogenetic analysis of the combined pcox1 and pnad1 mt DNA sequences were able to distinguish M. orientalis from different species of the Opisthorchiidae and trematodes in other families. Therefore, the ITS, cox1 and nad1 mt DNA sequences provide effective genetic markers for the specific identification of trematodes of the Opisthorchiidae family and have implications for studying their population biology, genetic structure, as well as molecular epidemiology.
  Y.Z. Cheng , J. Hou , X.H. He , Z.K. Hong , L.S. Li , G.H. Lin , M.X. Chen and S.H. Chen
  Paragonimiasis and angiostrongyliasis are important food-borne parasitic diseases in a number of countries including China. Both diseases are transmitted by freshwater and terrestrial intermediate snail hosts. In the present investigation, we examined the prevalence of Paragonimus and Angiostrongylus cantonensis in the intermediate host snails in southeastern China’s Fujian province where paragonimiasis and angiostrongyliasis are endemic. The prevalence of P. westermani cercariae in the stream-type snail Semisulcospira libertine found in the breeding grounds in Shouning county was 0.09% in the upstream and 0.56% in the downstream, respectively. For pit-ditch type, the prevalence of P. westermani cercariae was 0.19% and 0.82% in upstream and downstream, respectively. The snail Tricula xiaoqiaoensis was infected with Paragonimus skrjabini cercariae with prevalence of 0.33% in the pit-ditch type and 1.52% in the seepage type in Zhenghe county. Prevalence of A. cantonensis larvae in Achatina fulica in Nan’an county in garbage stack, vegetable plot and crop land were 83.54, 39.53 and 19.23%, respectively. In Lianjiang county, prevalence of A. cantonensis larvae in snails Pila polita in trench, paddy field, residential ditch, vegetable irrigation ditch and pond in the same river system were 4.08, 8.82, 75.34, 34.04 and 5.56%, respectively. The snail Bellamya lithophaga found in the western suburb of Fuzhou city was identified as the new intermediated host for A. cantonensis with a prevalence of 14.38%. Demonstration of prevalence of Paragonimus and A. cantonensis in wild snails in Fujian province poses substantial risk for future outbreaks of the two food-borne parasitic diseases.
  S.H. Chen , I. Ai , Y.C. Cai , Y.N. Zhang , G.S. He and J.X. Chen
  Cryptosporidiosis in animals is always with the symptom diarrhea which is not easily diagnosed and even be overlooked. Currently, researchers present here a case of this disease in Baird’s tapir with watery diarrhea in Shanghai wild zoo. The morphology identification, fecal antigen and PCR Method have been used in the detection. Moreover, all the testing assaies have shown coincidental result and species of Cryptosporidium has been ascertained as C. suis by sequencing. Therefore, the three examination methods may be useful tools for the diagnosis of cryptosporidiosis.
  H. Li , S.H. Chen , Y.N. Zhang , L. Ai and J.X. Chen
  Fish tapeworm (Diphyllobothrium species) are endemic in many parts of the word, the commonest species being Diphyllobothrium latum. Human are the main definitive host of D. latum and the majority of individuals harbouring the parasite are asymptomatica. In this report, researchers describe a case of D. latum infection founded on routine detection of eggs in fecal. After the whole cestode gotten, PCR Molecular Method had been done for identification.
  L. Ai , M.X. Chen , S.H. Chen , Y.N. Zhang , H. Li , Y.C. Cai , Y. Lu , L.G. Tian , X.N. Zhou and J.X. Chen
  MicroRNAs (miRNAs) are small non-coding RNA which generate from large hairpin precursors and play important role on post-transcriptional regulators of target genes. Although, many individual miRNAs have recently been extensively studied, there has been very little research on miRNA transcriptomes in trematode. By the method of using high throughput Solexa sequencing technology, 11.02 million clean reads have been obtained of Paragonimus westermani. Among the clean reads, 1.14 million ones (10.37%) were perfectly mapped onto the S. japonicum genome which included 20,322 (0.69%) unique sequences. A number of 9,492,613 (86.17%) reads had no match with public databases and marked as un-annotated RNAs. Nucleotide bias analysis found that the known miRNAs showed high bias and the guanine was the dominant nucleotide, particularly at the 2nd and 23rd positions which were almost at the beginning and end of conserved miRNAs. Three novel miRNA corresponding to 18 precursors were predicted. To the knowledge, this is the first report of miRNA profiles in P. westermani which will contribute to better understanding of the complex biology of this zoonotic parasite. The reported data of P. westermani miRNAs should provide valuable references for miRNA studies of closed related zoonotic trematode.
  Y.H. Chu , J.X. Chen , L. Ai , Y.C. Cai , Y.L. , J. Li , G.S. He and S.H. Chen
  Cryptosporidiosis is an important gastrointestinal disease in snakes. In the current study, 143 feces samples of cobra snakes and Oriental rat-snake were examined for the presence of cryptosporidia by morphology and Polymerase Chain Reaction (PCR) Method targeting a part of the 18S ribosomal RNA gene. A consecutive sequencing reaction was used to identify the cryptosporidian species present in PCR-positive samples. The oocyst of Cryptosporidium serpentis was found in 19 out of 143 (13.29%). The results stress the importance for diagnostic methods to be specific for Cryptosporidium species especially in snakes.
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