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| Articles
by
S.H. Chang |
Total Records (
1 ) for
S.H. Chang |
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O. Baatartsogt
,
K. Choi
,
P.K. Mandal
,
H.K. Lim
,
J.D. Oh
and
S.H. Chang
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This research was done to study the protein expression
profile of synovial cells of knee using proteome analysis during the development
of experimental Post-Traumatic Arthritis (PTA) in swine. PTA was induced
by transection of the Anterior Cruciate Ligament (ACL) of left knee in
three piglets. Articular cartilage and synovial tissues were obtained
after 0, 2, 5 and 8 weeks for histopathologic examination. After sacrificing
the piglets at 8 weeks, synovial tissues were collected for 2-dimensional
electrophoresis and mass spectrometric analysis. Histopathologic examination
at 8 weeks showed overt chronic inflammation indicating the development
of ETA. Through proteome analyses more than 1,500 protein spots were identified
in which 7 differentially expressed protein spots were observed in ACL-transacted
synovial tissue. Five proteins were down-regulated (cytoskeletal β
actin, cofilin-1, destrin, Rho GDP dissociation inhibitor α and an
unnamed protein product) and two proteins were up-regulated (α-B
crystalline and Smooth Muscle Protein (SMP) 22α). These results showed
that proteins that are related to cellular organization and signal transduction
are down-regulated and those that are related to cell rescue, defense
and stress are up-regulated. Therefore, the proteome analysis of synovial
tissue provided us new candidate molecules which may be useful to understand
the pathogenesis for diagnostic and therapeutic studies of Post Traumatic
Arthritis (PTA). |
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