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Articles by S.F. Youssef
Total Records ( 4 ) for S.F. Youssef
  N.A. Selim , S.F. Youssef , A.F. Abdel-Salam and Sh.A. Nada
  This study was conducted to evaluate the efficiency of aqueous extract of ginger root (GAE), aqueous extract of beetroot (BAE) and tomato puree (TP), as natural antioxidant sources, in broiler diets during summer season. Chicks fed on corn-soybean meal basal diets in three phases feeding system. Basal diets were formulated to contain whole strain nutritional requirements including 50 IU of vitamin E/kg. There were two control groups the first fed on basal diet (Con) and supplemented with either 50 IU/kg vitamin E (E-100, to be the second control group), or supplemented with GAE, BAE or TP at levels of 0.5 or 1% from 1 to 40 d of age. The total phenols content (in Gallic acid equivalent) of GAE and BAE were 44 and 31 μg/L, respectively and lycopene content in TP was 155 mg/kg. The environmental temperature and humidity surrounding birds during the experimental period ranged from 35 to 41°C and 30 to 45%. Growth performance, some plasma constituents and viral and bacterial immunity status at 40 d of age were recorded. The results indicated that, among examined natural additives, using BAE improved the overall body weight gain, while adding GAE to broiler diets decreased feed consumption significantly. However the worst feed conversion ratio recorded by TP group, that group recorded the best antioxidant status including plasma total antioxidant capacity and malondialdehyde. Generally, level 1% of supplementation increased the consumed feed and depressed the total bacteria count of intestine at 40 d of age. On the other side, titer of immunity against respiratory viral Diseases (ND and IB), hemoglobin, red and white blood cells increased by adding GAE to diets. Neither antioxidant source, level nor their interactions could change lipids or cholesterol compounds in plasma. The overall results indicated that the best overall broiler performance during summer season was obtained by using GAE at level of 1% as natural antioxidant.
  N.A. Selim , Sh.A. Nada , A.F. Abdel-Salam and S.F. Youssef
  This study was conducted to evaluate the efficiency of aqueous extract of ginger root (GAE), aqueous extract of beetroot (BAE) and tomato puree (TP) as natural antioxidant sources in broiler diets during summer season. Three hundred twenty 1-d-old Arbor Acres broiler chicks (mixed sex) were randomly allocated into 32 wire cages that were then randomly divided into 8 groups (treatments, 4 cages per treatment). Chicks fed on corn-soybean meal basal diet (Con, contained 50 IU of Vit. E/Kg) supplemented with either 50 IU /Kg vitamin E (E-100) or one of the additives (GAE, BAE and TP) at levels of 0.5 or 1.0% from 1 to 40 d of age. The total phenols contents (as Gallic acid equivalent) of GAE and BAE were 44 and 31 μg/L, respectively and lycopene content in TP was 155 mg/Kg. At 40 d of age, 32 birds (4 birds/treatment) were slaughtered and carcass characteristics were recorded. Refrigerated (up to 4 days at 4°C) and frozen (60 days at -20°C) meat samples were examined for total phenols content, antioxidant activity (through two scavenging assays: DPPH and TBARS) and microbiological status. The obtained results showed that neither antioxidant source nor level affected on dressing %, thickness of breast meat, triglycerides and cholesterol compounds of chilled breast meat. On the other side, source of antioxidant have affected strongly on oxidative stability especially after freezing for 60 d. Among examined sources, using BAE was less efficient in reducing oxidation rate than both GAE and TP. The microbiological examination showed strong effect of both tested sources and levels of natural antioxidant additives on decreasing count of total bacteria and Staph. aureas in refrigerated and frozen broiler meat. Using BAE failed to decrease count of Staph. aureas in frozen meat as GAE and TP. According to results of DPPH and TBARS assays and microbiological examination, adding GAE or TP to broiler diets could protect meat safe and healthy even with prolonged storage by freezing to 60d.
  N.A. Selim , N.L. Radwan , S.F. Youssef , T.A. Salah Eldin and S. Abo Elwafa
  This study was conducted to evaluate the efficiency of different selenium (Se) sources and levels in corn-soybean meal broiler diets. For that, 400 day-old unsexed Arbor Acres broiler chicks were allocated to 10 experimental treatments in a 5 (sources) x 2 (levels) factorial arrangement. Five Se sources were tested; (1) sodium selenite (NaSe) as inorganic form; (2) selenomethionine (Se-Yeast) as organic form; (3) Zinc-L-selenomethionine (Zn-Se-Meth) as more recent organic form; (4) powder form of Nano Se form (P-Nano Se) and (5) Liquid form of Nano Se (L-Nano Se). Also two inclusion of Se levels in diets; 0.15 and 0.30 ppm, were examined. The inorganic and organic forms of examined Se were obtained from commercial suppliers while both powder and liquid forms of Nano Se were prepared immediately before starting feeding phases of the experiment. The prepared 80 nm Se nano-particles were synthesized by chemical reduction method and characterized by Transmission Electron Microscope, X-ray diffraction and spectrophotometry. Three phases (1-10, 11-24 and 25-40 d) of feeding were applied and all birds were kept under similar management conditions. Parameters of growth performance, carcass characteristics and concentration of Se in both liver and thigh muscles were investigated. Also assay of Malnodialdhyde (MDA) was carried out in frozen (6 months at -20°C) thigh muscles to investigate the oxidation status of broiler meat. The obtained results showed significant improvement of growth performance and Se concentration in liver and thigh tissues either due to using organic or nano forms of Se, or by increasing the inclusion Se level from 0.15 to 0.30 ppm in broiler diets. While carcass abdominal fat%, giblets% and MDA content in thigh muscles did not affected due to Se sources or levels. Liver showed grater Se concentration than thigh muscles. The overall experimental results showed that using Se-Yeast or Zn-Se-Meth as organic forms of Se, or L-Nano Se as nano form of Se at level of 0.30 ppm in broiler diets or its equivalent in drinking water, respectively, is more effective to get better growth performance and quality of broiler meat. But further studies about the safety of using nano form of selenium as feed additives are needed.
  N.A. Selim , N.L. Radwan , S.F. Youssef , T.A. Salah Eldin and S. Abo Elwafa
  This study was conducted to evaluate both physiological and immunological efficiency and toxicological effects of different selenium (Se) sources and levels in corn-soybean meal broiler diets. For that, 400 day-old unsexed Arbor Acres broiler chicks were allocated to 10 experimental treatments in a 5 sources x 2 levels factorial design. Five Se sources were tested; (1) sodium selenite (NaSe) as inorganic form; (2) selenomethionine (Se-Yeast) as organic form; (3) Zinc-L-selenomethionine (Zn-Se-Meth) as more recent organic form; (4) powder form of Nano Se form (P-Nano Se) and (5) Liquid form of Nano Se (L-Nano Se). Also two inclusion of Se levels in diets; 0.15 and 0.30 ppm, were examined. The inorganic and organic forms of Se were obtained from commercial suppliers while both powder and liquid forms of Nano Se were prepared immediately before starting feeding phases of the experiment. The prepared 80 nm Se nano-particles were synthesized by chemical reduction method and characterized by Transmission Electron Microscope, X-ray diffraction and spectrophotometry. Three phases (1-10, 11-24 and 25-40 d) of feeding were applied and all birds were kept under similar management conditions. Parameters of blood picture, immunity status, antioxidant status, some plasma constituents and T3 hormone were investigated. Also histological examination of liver samples was carried out at 40 days of age. The obtained results showed significant improvement of some hematological parameters, cellular immunity and antioxidant status either due to using organic or nano forms of Se, or by increasing the inclusion Se level from 0.15 to 0.30 ppm in broiler diets. While humeral immunity against Newcastle Disease Virus and Avian flow Virus (H5N1), plasma proteins, activity of liver enzymes and malnodialdhyde (MDA) content in plasma did not affected due to Se sources or levels. Concentration of T3 hormone significantly increased by increasing Se level from 0.15 to 0.30 ppm in the diet. The histological examination of liver showed some severe pathological changes due to increasing Se level from 0.15 to 0.30 ppm for most sources while using 0.15 ppm of Se from inorganic or organic forms of Se showed normal histological structure of liver tissues. The overall experimental results showed although using Zn-Se-Meth as organic form of Se or L-Nano Se as nano form of Se or increasing the supplemental Se to 0.30 ppm in broiler diets or its equivalent in drinking water is more effective to get better, physiological, immunological and antioxidant status of broiler chicks. Inclusion Se-Yeast as organic form of Se in broiler diets at level 0.15 ppm was more save to liver tissues and kidney function. Further studies about the safety of using nano form of selenium as feed additives are needed.
 
 
 
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