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Articles by S.E. Atawodi
Total Records ( 2 ) for S.E. Atawodi
  A.O. Ogbe , U. Ditse , I. Echeonwu , K. Ajodoh , S.E. Atawodi and P.A. Abdu
  Proximate and chemical composition of a wild mushroom, Ganoderma sp was evaluated. The phytochemical analysis showed it contained carbohydrates and reducing sugars, steroids, cardiac glycosides, saponins and resins. Proximate analysis revealed crude protein (13.3%±0.2), crude fibre (34.7%±6.4), fats (2.6%±0.3), calcium (0.4%±0.1) and phosphorus (0.3%±0.0). Acidic amino acids (glutamic and aspartic acid) (6.2 g%±1.4 and 5.6 g%± 0.1) and sulphur containing amino acids (cystine and methionine) (1.5 g%±1.3 and 0.7 g%±0.1) were also detected. Of all the essential amino acids detected in the mushroom, leucine was higher (5.3 g%±0.9), followed by phenylalanine (3.7 g%±0.5), alanine (3.3 g%±0.1), isoleucine (3.1g%±0.1), valine (3.0 g%±0.2), lysine (3.0 g%±0.6) and threonine (2.0 g%±0.1). Histidine (1.7 g%±0.3) and methionine (0.7 g%±0.1) were the least. Supplemented diets showed higher leucine content followed by phenylalanine, alanine, isoleucine, valine, lysine, threonine, histidine and methionine. Pullets fed these diets showed improved performance in weight gain and health. The physical qualities of eggs laid by the birds were not affected. Although, feed intake did not show significant difference in all the groups (p>0.05) but the feed to gain ratio was better in A (3.3) and B (3.4) than C (3.5) and D (3.6). This showed supplementation with the mushroom resulted in better feed efficiency and the effect is dose dependent. It was concluded that this mushroom can be a valuable source of feed supplement to improve performance and health.
  A.C. Ene , S.E. Atawodi , D.A. Ameh , H.O. Kwanashie and P.U. Agomo
  The possibility of developing experimental chloroquine resistant Plasmodium berghei NK65 from chloroquine sensitive Plasmodium berghei NK65 was evaluated. Five mice of about 12 weeks old were inoculated with Plasmodium berghei (CQ sensitive strain). Exactly 72 h after inoculation and confirmation of parasitemia, these mice were treated with 10 mg kg-1 body weight (b.wt.) every 48 h for one month. After this period, treatment was withdrawn for one week, following which sub-inoculation was made from each of the five mice to four new mice for each group respectively. Seventy two hours after parasitemia was confirmed in the sub-inoculated mice, two of the four mice in each group were treated with the correct dose of chloroquine, that is, 25 mg kg-1 b.wt. daily for four days, while the rest were not treated. Parasitemia was monitored in all the groups for two weeks using thick and thin smears of blood films made from the tail vein of mice and stained with 10% Giemsa stain at pH 7.2. Two weeks after treatment with 25 mg kg-1 b.wt. dose of chloroquine was stopped, four mice died in the first two groups, while one mouse each died in the remaining three groups. Six of the untreated mice from the replicate groups equally died beyond two weeks, while four survived. At death, the % parasitemia of mice that died were higher than those that survived after 2 weeks. These results suggest that those mice that survived two weeks after treatment with the right dose of chloroquine (25 mg kg-1 b.wt. for 4 days) contained chloroquine sensitive Plasmodium berghei NK65 before they were cleared, while those that had persistence of parasitemia at relatively high level which resulted in their death contained chloroquine resistant Plasmodium berghei NK65. This finding should be of importance in studies involving development of new therapy for chloroquine resistant malaria.
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