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Articles by S.D. Siadat
Total Records ( 2 ) for S.D. Siadat
  F. Pourfallah , S. Javadian , Z. Zamani , S.D. Siadat , Sh- Khatami , R. Saghiri and T. Kordi
  This study was a cross-sectional descriptive study and done in four seasons during April 2011 to March 2012.The objective of the present study was to examine Physico-chemical properties of groundwater around Tehran. The results are also compared with the guideline values of Iranian legislation. A total of 160 drinking water samples were collected from different drinking groundwater around the Tehran. Total Dissolved Solids (TDS), conductivity and pH, were measured by using standard methods and the concentration of ions Cl¯, F¯, NO3¯, NO2¯, Br¯, SO42¯, PO43¯, Ca2+, K+, Na+ and NH4+ in groundwater was performed using Ion chromatography (Metrohm Company, USA) with standard method. This study showed that most of the parameters in groundwater were below the Iranian permissible limit except total dissolved solids (N = 2), conductivity (N = 2), nitrate as NO3¯ (N = 22), chloride (N = 3), sulphate (N = 2), fluoride (N = 3) and ammonia (N = 8). There were significant differences (p<0.05) between physico-chemical parameters such as pH, nitrite (NO2¯), sodium, potassium, sulphate, ammonia, bromide and phosphate in different seasons. These results are important, not only for the many people who drink groundwater but also for the health supervisory agencies such as Ministry of Health and Institute of Standards and Industrial of Iran (ISIRI) to have more effective control on groundwater.
  Q. Behzadiyannejad , S.D. Siadat , M. Kheirandish , B. Tabaraiee , H. Ahmadi , D. Norouzian , S. Najar Peerayeh , M. Nejati and M.H. Hedayati
  Production of effective vaccine formulations is dependent on the availability of assays for the measurement of protective immune responses. Antibody- and complement-mediated phagocytosis is the main defense mechanism against Neisseria meningitidis. Therefore, a newly developed phagocytosis assay based on flow cytometry (flow assay) and the Serum Bactericidal Activity (SBA) assay were using sera obtained from rabbit postvaccination with the Outer Membrane Vesicles (OMVs) of Neisseria meningitidis serogroup B was done in order to evaluation of the potential efficacy of (experimental) meningococcal vaccines. The OMVs were injected intramuscularly into of rabbits with boosters on days 14, 28 and 42 after the primary immunization. Phagocytic function of and intracellular oxidative burst generation by rabbit PMN, against Neisseria meningitidis serogroup B, was measured with flow cytometer (Coulter Epics-XL-Profile USA), using dihydrorhodamine-123 as probes, respectively. SBA titers are given as reciprocal Log 2 values of the dilution giving at least 50% killing of the inoculum measured as colony forming units. The results of SBA titers and quantitative flow cytometric analysis of rabbit PMN function in hyperimmun sera with the OMVs revealed a highly significant increase in opsonophagocytic responses and bactericidal antibody against serogroup B meningococci after 56 day (p< 0.05). Both SBA and opsonic activity are crucial for the protection against meningococcal disease. In conclusion, we have shown a very high correlation between opsonic activity and SBA (r = 0.91). Present results indicated that the OMVs could be as a candidate for vaccine toward serogroup B meningococci.
 
 
 
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