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Articles by S.D. Karou
Total Records ( 4 ) for S.D. Karou
  E.F.O. Akomo , C. Zongo , S.D. Karou , L.C. Obame , A. Savadogo , C. Atteke and A.S. Traore
  Because of the resistance of pathogens to actually available drugs, there is a continuous need for new agents to cure several diseases such as malaria and microbial infections. In the establishment of new drugs, plant are often a source of new leads, indeed aqueous, acetone and methanol extracts of Canthium multiflorum were tested for antiplasmodial and antibacterial activities. The antiplasmodial activity was performed on fresh clinical strains of Plasmodium falciparum using light microscopy. The results revealed that the methanol extract was the most active with IC50 of 4.69 μg mL-1. The NCCLS microdilution method performed on clinical and reference strains of pathogenic bacteria yielded MIC and MBC values ranging from 312 to 1250 and 625 to 2500 μg mL-1, respectively. The qualitative analysis of the extract revealed the presence of several chemical groups such as alkaloids, terpens and tannins that might be responsible for the activity of the plant. The issue of this study showed that C. multiflorum is a plant that many attention should be paid to because of it pharmacological potentials.
  L.W. Nitiema , D. Dianou , J. Simpore , S.D. Karou , P.W. Savadogo and A.S. Traore
  An anaerobic digester fed with shea cake rich in tannins and phenolic compounds rich-shea cake and previously inoculated with anaerobic sludge from the pit of a slaughterhouse, enabled six months acclimatization of the bacteria to aromatic compounds. Afterwards, digester waste water samples were subject to successive culture on media with 1 g L-1 tannic acid allowing the isolation of a bacterial strain coded AB. Strain AB was facultatively anaerobic, mesophilic, non-motile, non-sporulating, catalase and oxidase negative bacterium, namely strain AB, was isolated from an anaerobic digester fed with shea cake rich in tannins and phenolic compounds, after inoculation with anaerobic sludge from the pit of a slaughterhouse and enrichment on tannic acid. The coccoid cells occurred in pair, short or long chains and stained Gram-positive. Strain AB fermented a wide range of carbohydrates including glucose, fructose, galactose, raffinose, arabinose, sucrose, maltose, lactose, starch and cellulose. Optimum growth occurred with glucose and tannic acid at 37°C and pH 8. The pH, temperature and salt concentration for growth ranged from 5 to 9, 20 to 45°C and 0 to 15 g L-1, respectively. Strain AB converted tannic acid to gallic acid. These features were similar to those of the Streptococcus genus. The determination of tannic acid hydrolysis end products, ability to utilize various organic acids, alcohols and peptides, GC% of the DNA, the sequencing of 16S rRNA gene and DNA-DNA hybridization will permit to confirm this affiliation and to determine the species.
  T. Sagna , F. Djigma , M. Zeba , C. Bisseye , S.D. Karou , D. Ouermi , V. Pietra , C. Gnoula , K. Sanogo , J.B. Nikiema and J. Simpore
  The vaginal swabs among HIV-positive women in Africa often revealed opportunistic infections such as human Papillomavirus (HPV) and Mycoplasma that induce respectively cervix cancer and diseases such as vaginosis, abortions, infertility in through salpingitis. The purposes of this study were to: (1) seek for, the prevalence of pathogens such as HPV and Mycoplasma; (2) characterize the strains of HPV and estimate their prevalence; (3) identify among these women, those who were co-infected by these pathogens in order to cure them. From February 2009 to January 2010, 156 HIV-positive women attending our medical centers and aged from 19-45 years (mean age 33.65±5.75 years) had voluntarily accepted vaginal specimen’s tests. PCR, ELISA and molecular hybridization were used for the identification and characterization of these pathogens. The results revealed the presence of Mycoplasma and HPV in 25.64 and 58.33% cases, respectively. The following HPV genotypes and the following prevalence were recorded: HPV-50'S (24.11%), HPV-18 (21.28%), HPV-30'S (18.44%) and HPV-16 (5.67%). The study also enable the identification of co-infections such as HPV-18 strains with HPV-30'S (5.67%) and HPV-30'S with HPV-50'S (3.55%). Other germs infecting the female genital tract including Candida albicans (20.51%), Escherichia coli (12.18%), Treponema pallidum (3.85%), Streptococcus agalactiae (3.21%) and Staphylococcus aureus (1.92%) were isolated. This preliminary research work showed the incidence of several genital pathogens, this could be a springboard for nationwide epidemiological study on HPV strains circulating in Burkina Faso.
  M.T.A. Zeba , C.A.T. Ouattara , S.D. Karou , C. Bisseye , D. Ouermi , F.W. Djigma , T. Sagna , V. Pietra , R. Moret , J. Nikiema and J. Simpore
  HCV and HBV cause annually, 2000 deaths from liver cancer in Burkina Faso. In this country, serological screening of hepatitis viruses B and C is only systematic among blood donors. The aims of this study were; (1) to investigate the reasons for the prescription of the screening for hepatitis B and C; (2) to determine HCV and HBV prevalence among 462 patients attending the Saint Camille Centre and (3) to identify patients with acute hepatitis or with chronic hepatitis for better monitoring. From February to May 2012, 462 patients attending the laboratory of the Saint Camille Medical Centre with viral hepatitis suspicion were screened. The hepatitis B and C serological markers were detected through Enzyme Immuno Assay (EIA) technique using commercial reagent kits. The clinical symptoms were also recorded for each patient. The results revealed that, the main clinical symptoms that prompted physicians to request HBV and HCV screenings were: asthenia (39.4%), anorexia (21.2%), abdominal pains (19.0%), nausea (10.4%), others (10.0%). The prevalence of HbsAg was 29.4% among the screened people. Patients with acute hepatitis B, active chronic hepatitis B and non-active chronic hepatitis B represented 11.2, 2.2 and 16.0%, respectively. The acquisition of immunity against HBV after vaccination was attempted for 11.7% people. HCV prevalence was 3.9% and its coinfection with HBV was 2.2%. This study showed a high prevalence for hepatitis B and C among patients attending Saint Camille Medical Centre. Without hygiene education and HBV/HCV prevention, viral hepatitis infection will become a serious public health problem in Burkina Faso.
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