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Articles by S.D. Ahmad
Total Records ( 2 ) for S.D. Ahmad
  L. Hassan , S.D. Ahmad and A. Okumus
  The genetics of plants regenerated from anther-derived callus was investigated using single crosses & inbreds of maize. Anther culture in maize (Zea mays L.) usually follows an indirect procedure involving callus induction and differentiation on at least two different media. Alternatively, development of a direct procedure by which plantlets can be regenerated directly on one medium seems desirable, if acceptable frequency of plant regeneration could be obtained and an effective procedure of chromosome doubling of regenerated plantlets is available. Yupie (YP) and N6 were used as basal media and 10 growth regulators (2,4-D, kinetin, NAA, IAA, IBA, dicamba, pCPA, 6-BA, 2ip and hypoxanthine) were tested to evaluate the feasibility of direct regeneration of haploid plantlets of eight single cross hybrids and four inbred lines. The results indicated that the direct generation of plantlets is feasible through anther culture, though it is genotype and medium dependent. Ten plantlets were regenerated directly from single cross hybrid M60 X M96. The more effective medium was N6 basal medium plus 2,4-D (2.0 mg l-1) and kinetin (1.5 mg l-1). Regeneration frequency (percent of anthers producing plantlets) ranged from 1 to 4%.
  L. Hassan , S.D. Ahmad , A. Okumus and M. Wazuddin
  A 3 hr. period treatment with a 0.2% aqueous solution of colchicine was given to one week old seedlings of seven inbred lines of wheat (Triticum aestivum L) plants. Comparisons were made in the CT1 generation between the untreated controls (2x) and colchicine treated plants (C2x) for variation of leaf mesophyll cell size and chloroplast number. Differences were found in that the cell section area and the number of chloroplasts per cell in all the seven lines investigated were significantly greater in the C2x selection compared with the 2x controls using coded determination. Heritable differences were found such that the cell selection areas and the numbers of chloroplasts per cell were significantly greater in the C2x treatment in all the lines. In each case there was an enhancement of chloroplast number independent of the variation in cell size.
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