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Articles by S.A. Adeola
Total Records ( 3 ) for S.A. Adeola
  S.A. Adeola , O.S. Folorunso , O.O. Okedeyi , B.F. Ogungbe , O.B. Babatimehin and O.Z. Thanni
  The emergence of antibiotic resistant bacteria has made the treatments of pathogenic infections difficult. The discovery that some analytes of plant extracts are active against multi-drug resistant bacteria has opened ways to researching into the antimicrobial activity of these phytocomponents. Studies are designed to determine the antimicrobial and antioxidant potentials of the volatile oil of Ocimum gratissimum and its inhibition on partially purified and characterized extracellular protease of Salmonella enteritidis. The oil was extracted by hydrodistillation. Its antimicrobial effect was carried out using microdilution method while antioxidant effect was tested against 1,1-diphenyl-2-picrylhydrazyl (DPPH). The caseinolytic activity of the enzyme was studied with oil as inhibitor. The enzyme was partially purified with dialysis and gel filtration. The oil has scavenging activity of 66.98±1.78% compared to butylated hydroxyltoluene (positive control), 53.29±2.51% (sig. p<0.0001) against DPPH. Salmonella enteritidis was inhibited by this oil with IC50 of 3.98% (v/v). The enzyme had optimal activities at 45°C and pH 7.5. None of the metallic chloride tested produced any significant increase in the activity of the enzyme. Hg2+ and Pb2+ were inhibitors of this enzyme. The oil showed a non-competitive inhibition with Km = 0.33 mg mL-1, Vmax = 1.25x103 μmol min-1 (oil absent) and V’max = 2.50x102 μmol min-1 (oil present). The highest purification fold = 1.88 and the highest percentage yield = 51.74 as compared to the crude enzyme extract. Therefore, the volatile oil of Ocimum gratissimum possessed antimicrobial and antioxidant activities, its inhibition against this protease may be one of the ways of its antimicrobial effect.
  S.A. Adeola , O.S. Folorunso , G.O. Raimi and A.B. Akinsiku
  The recent development in the antimicrobial therapy to meet the challenges of resistant pathogenic bacteria has called for rapid growth in exploring natural products as alternative. The antimicrobial activity of the oils from the leaf and stem of Chromolaena odorata against nine enteric pathogenic bacteria was assessed and compared with commonly used antibiotics. Specifically, we examined the mode of inhibition of these oils on the extracellular protease of Pseudomonas aeruginosa. The inhibition zones of the oils range from 13.0±1.0-43.5±2.5 mm in Salmonella paratyphimurium and Shigella dysenteriae, respectively. Ceftriaxone has the highest inhibition of 26.0±2.0 mm against Salmonella paratyphimurium. There was a significant difference (p<0.05) between the average inhibition of the antibiotics, 5.0±0.82 mm and the volatile oils, 18.0±4.0 mm. Each of the microbes was sensitive to at least one of the oils. The extracellular protease of Pseudomonas aeruginosa had optimal activities at pH 7.5 and 35°C. The volatile oils competitively inhibited the extracellular protease of Pseudomonas aeruginosa with Vmax = 0.91 μmol min-1 and Km = 0.48 mg mL-1 (oil absent) but the K'm increased to 0.93 and 1.25 mg mL-1 in the presence of the volatile oils of the leaf and stem of this plant, respectively. The highest purification fold of 2.35 (6.92 μmol min-1 mg-1 protein) was achieved from the crude enzyme with DEAE anion exchange chromatography. The chromatographic peaks may be an evidence of multiple subunits of this enzyme. Therefore, the oil of Chromolaena odorata possessed antimicrobial activity and its ability to inhibit extracellular protease of Pseudomonas aeruginosa will be a possible source of nutraceuticals for clinical purpose.
  M.I. Kazeem , T.V. Dansu and S.A. Adeola
  In recent decades, there has been a drastic increase in the incidence and prevalence of diabetic mellitus. The aim of this study was to evaluate the in vitro inhibitory effect of Azadirachita indica leaf extract on the activity of α-amylase and α-glucosidase as a means of alleviating hyperglycemia and managing diabetes mellitus. Aqueous extract of Azadirachita indica exhibited most potent α-amylase inhibitory activity with IC50 value of 9.15 mg mL-1 and acetone extract exhibited most potent α-glucosidase inhibitory activity with IC50 value of 5.00 mg mL-1. Kinetic studies revealed both acetone and aqueous extract to exhibit mixed non-competitive inhibition for α-amylase and α-glucosidase. It can be concluded that the antidiabetic potential of Azadirachta indica may be due to its ability to inhibit both α-amylase and α-glucosidase. The presence of phytochemicals such as flavonoids, tannins and saponins in this plant may be responsible for its inhibitory activity on the two enzymes studied.
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