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Articles by S.A. Mirhadi
Total Records ( 3 ) for S.A. Mirhadi
  S. Zakizadeh , G. Rahimi , S.R. Mirae-Ashtiani , A. Nejati-Javaremi , M. Moradi-Shahrbabak , P. Reinecke , M. Reissmann , A.A. Masoudi , C. Amirinia and S.A. Mirhadi
  The aim of this study was to estimate the allelic frequency in polymorphic sites of intron 3 and exon 5 of bovine growth hormone gene in three Iranian native and Holstein cattle. A total of 406 genomic DNA samples were extracted from three Iranian native cattle including, Mazandarani 97, Sarabi 87, Golpaygani 112 and Holstein 110 cattle. The PCR procedure was used to amplify 345 bp of bGH-intron 3 and 404 bp of bGH-exon 5. The frequencies of MspI (+) allele were estimated as 0.55, 0.51, 0.44 and 0.83 in Mazandarani, Sarabi, Golpaygani and Holstein breed, respectively. The allelic frequencies of AluI (+) were calculated as 0.91, 0.84, 0.92, 0.85 and for DdeI (+), 0.52, 0.54, 0.47 and 0.86, for these breeds, respectively. Chi-square test showed significant differences (p<0.01) in genotypic frequency between native and Holstein breed in MspI and DdeI restriction sites. There was significant differences in genotypic frequencies between Mazandarani (p<0.05) and Golpaygani (p<0.01) with Holstein breed at AluI restriction site. This difference was not significant between Sarabi and Holstein breed. The differences in allelic frequency between native breeds and Holstein cattle at the present study might be due to differences in origin of breeds, selection plans applied to Holstein population for improving milk production.
  M.R. Ebadi , J. Pourreza , J. Jamalian , M.A. Edriss , A.H. Samie and S.A. Mirhadi
  In order to assess and determine amino acids profile and availability in different sorghum grain (SG) varieties, three SG varieties including low tannin (0.09%, LTS), medium tannin (0.19%, MTS), and high tannin (0.37%, HTS) were grown in the same location. The grains were analyzed for their proximate analyses, tannin, and amino acids. True amino acid availability (TAAA) was obtained by caecectomized single comb leghorn cockerels and Sibbald`s method. The results showed that the first and the second limiting amino acids in LTS and MTS were methionine and cystine, respectively. Whereas in HTS, lysine (0.166%) was the first and methionine (0.176%) was the second limiting amino acid. Methionine contents in LTS, MTS, and HTS were 0.114%, 0.182%, and 0.176%, respectively. Although crude protein was lower in HTS (10%) than the other SG, histidine, methionine, and isoleucine were higher for HTS as compared to LTS and MTS. TAAA decreased when tannin content increased and all amino acids had a lower availability in HTS than in LTS and MTS (p<0.05). Methionine in HTS and LTS had the highest availability as had glutamic acid in MTS. The availability of proline was most affected by tannin, which was 91.55% for LTS, 84.82% for MTS, and 22.82% for HTS (p<0.05).
  Ali Nouri-Emamzadeh , A. Yaghobfar , A.A. Sadeghi , S.A. Mirhadi and M. Chamani
  The experiment was conducted to determine metabolizable energy contents of Soybean (SBM), Canola (CM) and Sunflower (SFM) meals by Conventional Addition Method (CAM) included with intact and caecectomized adult cockerels for 7 day: A 3 day pre-experiment and a 4 day experiment period. The values of SBM and CM obtained using intact cockerels were approximately 11 and 5% higher (p<0.05) than those used cecectomized cockerels, respectively. There was no significant effect of caecectomy on the measured values for SFM (p>0.05). Therefore, the effect of caecectomy on ME values in adult cockerels is dependent on the compositions of the meal assayed. However, caecetomised and intact cockerels had not differences for amount of EEL and ENL excretion.
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