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Articles by S. Yokota
Total Records ( 2 ) for S. Yokota
  M.Z. Karim , S. Yokota , M.M. Rahman , J. Eizawa , Y. Saito , M.A.K. Azad , F. Ishiguri , K. Iizuka and N. Yoshizawa
  The optimum sucrose concentration and pH level were estimated for in vitro shoot regeneration from callus of Aralia elata Seem in a Broad-leaved tree (BT) medium with different concentrations of sucrose (0.0-25.0 g L-1) and a wide range of pH levels (4.5-6.8). The highest rate of shoot production was achieved in a shoot-regenerating medium with 15 g L-1 sucrose and a pH of 5.8. Shoot multiplication and growth were significantly affected by sucrose. Shoots were not formed in a sucrose-free medium. When the pH was adjusted below or above 5.8, the growth rate of the shoot significantly decreased.
  M.Z. Karim , S. Yokota , M.M. Rahman , J. Eizawa , Y. Saito , M.A.K. Azad , F. Ishiguri , K. Iizuka and N. Yoshizawa
  A highly reproducible in vitro shoot regeneration system in Aralia elata Seem. using root explants was developed in this investigation. Multiple shoots were induced in vitro directly from root explants through adventitious shoot bud regeneration. The ability of root explants to produce shoot buds depended on the supplementation of Plant Growth Regulators (PGRs). Maximum multiplication of shoots (18 shoots per explant) was achieved in a Broad-leaved Tree (BT) medium supplemented with 1.00 μM 6-benzyl aminopurine (BAP). The best and healthiest rooting was observed in a BT medium supplemented with 2.00 μM μ-naphthaleneacetic acid (NAA). Regenerated plants were successfully acclimatized under ex vitro condition.
 
 
 
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