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Articles by S. Tavakoli
Total Records ( 4 ) for S. Tavakoli
  M.T. Beigi Nassiri , Z. Hamidi and S. Tavakoli
  Blood samples of Mazandaran native chickens were collected. A total of 90 genomic DNAs were isolated through optimized and modified salting-out procedure. The samples were used in Polymerase Chain Reaction (PCR) with 20 micro satellite markers. Amplified PCR-products with the markers were separated on 8% denaturing polyacrylamide gel. One locus (MCW216) was monomorphic. According to allele frequencies of 20 micro satellite sites, mean heterozygosity (H) and Polymorphism Information Content (PIC) were calculated. The number of alleles varying from 1 to 6 and an estimate of average heterozygosity excluding the monomorphic data was calculated as 0.5872. The average heterozygosity and PIC value calculated from data on polymorphic and monomorphic loci was 0.5579 and 0.4939, respectively. The results of the heterozygosity were consistent with that of PIC. Diversity estimates in this study are lower than the observed frequencies of heterozygotes reported in other species using micro satellite markers. Other parameters for intrapopulation variation and Hardy-Weinberg proportions were also considered. All the loci except MCW222 and MCW165 showed deviations from Hardy-Weinberg equilibrium (p<0.005). Some of micro satellite sites were highly polymorphic, so they were effective markers for genetic diversity analysis. These results could provide basic molecular data for the research on the germplasm characteristics of Mazandaran native chickens.
  M. T. Beigi Nasiri , F . Shokri , S. Esmaeil Khanian and S. Tavakoli
  Polymorphism of Isfahan native chickens were evaluated using ten microsatellite markers. Nine microsatellite loci were found to be polymorphic, but one of them was monomorph (MCW0216). All microsatellite loci deviate from the Hardy-Weinberg equilibrium. Heterozygosity and the Polymorphism Information Content (PIC) were calculated to determine the genetic variation. Of the nine polymorphism loci, actual and effective number of alleles (na, ne) per locus ranged from 2 to 5 and 2 to 3.9014, respectively. PIC has values between 0.3750 to 0.6972 per locus (except monomorphic locus) and average of polymorphism information content based on 10 microsatellite was estimated as 0.4897 for this population. The average of heterozygosity exclusive of monomorphic locus ranged from 0.5 to 0.7437 per locus and by attention of monomorphic locus was 0.5613 per population. In general, it can be concluded that Isfahan native chickens population has approximately low genetic diversity. Therefore designing breeding project needs attention to conserving the genetic diversity, so the genetic resources will be conserved as world’s national investments.
  M.T. Beigi Nassiri , Z. Biranvand , T. Hartatik , J. Fayazil and S. Tavakoli
  The part of the bovine genome which shows a superior action and explains the major part of variation of the economical production traits were known as QTL. PIT1 gene which is also termed hormone factor-1 is a pituitary-specific transcription factor which has responsible for pituitary development and hormone expression in mammals. The main function of PIT1 is for binding and trans-activity promoters of both Growth Hormone (GH) and Prolactin (PRL) gene. It was subjected to different molecular studies as a key role for understanding genetic variation in dairy cattle. This study was aimed to detect the polymorphism in a part of PIT1 gene, exon 6 in Najdi cattle. DNA was extracted from blood sample collected from 84 Najdi Cattle and submitted for Polymerase Chain Reaction (PCR) followed by digestion with HinfI restriction enzyme. Base on PCR-RFLP method, it found that the frequency of the A and B alleles of this gene for Najdi Cattle were 18.45 and 81.55%, respectively. The genotypic frequencies of AA, AB and BB were 3.57, 29.76 and 66.66%, respectively. χ2-test indicated that these two populations were at Hardy-Weinberg equilibrium. Thus, the selection base on these loci in the population is possible to be done.
  M. T. Beigi Nassiri , Z. Beyranvand , T. Hartatik , J. Fayazi and S. Tavakoli
  The part of the bovine genome which shows a superior action and explains the major part of variation of the economical production traits were known as QTL. PIT1 gene which is also termed hormone factor-1 is a pituitary-specific transcription factor which has responsible for pituitary development and hormone expression in mammals. The main function of PIT1 is for binding and trans-activity promoters of both Growth Hormone (GH) and Prolactin (PRL) gene. It was subjected to different molecular studies as a key role for understanding genetic variation in dairy cattle. This study was aimed to detect the polymorphism in a part of PIT1 gene, exon 6, in Najdi cattle. DNA was extracted from blood sample collected from 84 Najdi cattle and submitted for Polymerase Chain Reaction (PCR) followed by digestion with HinfI restriction enzyme. Base on PCR-RFLP method, the researchers found that the frequency of the A and B alleles of this gene for Najdi cattle were 18.45 and 81.55%, respectively. The genotypic frequencies of AA, AB and BB were 3.57, 29.76 and 66.66%, respectively.
 
 
 
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