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Articles by S. Tachibana
Total Records ( 5 ) for S. Tachibana
  Sulistiyani , S. Falah , W.T. Wahyuni , T. Sugahara , S. Tachibana and Syaefudin
  The research objective is to obtain the cellular mechanism(s) of Syzygium polyanthum leaves for its development into commercial herbal product for certain degenerative diseases. The approach was to conduct activity-guided fractionation of metabolite extracts based on the antioxidant and the apoptotic modulation activities using cultured cells. Antioxidant activity of the active fractions were screened using 1,1-diphenyl-2-picrylhydrazil (DPPH) free radical assay and thiobarbituric acid (TBA) assay. The cellular effects were investigated using cultures of HB4C5 human hybridoma and mouse colon 26 adenocarcinoma cells. The flavonoids extract which has the lowest IC50 value of 14.84 μg mL-1 for DPPH assay among other metabolites, was found to have significant cytotoxic effect on both HB4C5 and colon 26 cells. Specific assays for apoptosis using caspase 3-gene expression and annexin-V flow cytometry analyses, supported the effect of cell proliferation inhibition which was not due to apoptosis. Subsequent cell cycle analysis indicated that crude extract may induce cell cycle arrest of HB4C5 cells at G1 to S phase, whereas active compound fractions of flavonoids stacked the cell cycle at G2/M phase. These observations suggest that flavonoid extracts of Syzygium polyanthum posses active compound(s) with anti-proliferative effect. This is a new finding for therapeutic potential of Syzygium polyanthum leaves in Indonesian traditional medicines.
  R.A.A. Lelono , S. Tachibana and K. Itoh
  Gardenia jasminoides E. (Rubiaceae) methanol extracts showed the highest level of antifungal activity against Pleurotus ostreatus, a wood-rotting fungus, compared to five other methanol plants extracts; [Thuja orientalis L. (Cupressaceae), Datura innoxia (Solanaceae), Ligustrum japonicum T. (Oleaceae), Juniperus chinensis var. procumbens (Cupressaceae) and Mallotus japonica M. (Euphorbiaceae)] and selected for further analysis. Two antifungal compounds were isolated from n-butanol and ethyl acetate solubles in the methanol extracts of Gardenia jasminoides leaves and stems by bioassay-guided fractionation, using Pleurotus ostreatus. The antifungal compounds found for the first time in Gardenia jasminoides against Pleurotus ostreatus were identified as genipin and geniposide based on instrumental analyses. Both also had potent inhibitory effects on two plant pathogenic fungi; Fusarium oxysporum and Corynespora cassiicola.
  Djarwanto and S. Tachibana
  In an effort to prevent forest fires after the clear cutting of plantation forests, fungi capable of degrading lignocelluloses were isolated to make a fertilizer from the logging waste. Seventy five fungal species were isolated from fruiting bodies and mycelia in plantation forests of South and North Sumatera, Indonesia. Sixty three of the fungi were identified based on the appearance and morphological characteristics of their fruiting bodies and mycelia, as Pycnoporus sanguineus, Dacryopinax spathularia, Schizophyllum commune, Polyporus sp. and Trametes sp. Twenty fungi were categorized as white-rot fungi and 12 as brown-rot fungi. Moreover, isolates 371, 368, 265, 346, 345 and 338 were selected using indicators and tested for the ability to degrade lignin and holo-cellulose in mangium wood meal over 1 to 4 weeks. Results showed that the 6 fungi could degrade lignin and holo-cellulose in wood meal. An increase in incubation time tended to decrease the amounts of lignin and holo-cellulose. Isolate 371 was found to be best at degrading lignin and holo-cellulose in mangium wood meal.
  Djarwanto and S. Tachibana
  This research was conducted in the aim of preventing wild fire through reducing potential energy source to become in situ fertilizer. To prevent forest fires by reducing wood waste using lignocellulose-degrading fungi, six fungal isolates were tested for lignin and cellulose-degrading activity with Acacia mangium leaves and twigs over a period of 1 to 3 months. The fungi degraded 8.9-27.1% of the lignin and 14-31% of the holocellulose. The degradation rate varied depending on the fungal species. An increase in incubation time tended to decrease the amounts of holocellulose and lignin. However, the hot water soluble tended to increase following a longer incubation period. From the results obtained here, more time was needed to degrade lignin rather than other components in the sample.
  N. Artanti , S. Tachibana , L.B.S. Kardono and H. Sukiman
  Endophytic microbes are considered as an important source of natural products. They show antibiotic, anticancer, antioxidative and antidiabetic activities. Therefore, there are many reports on the isolation and bioactivity screening of endophytic fungi from various plants including Taxus species. Taxus sumatrana (Miq.) de Laub is found in Indonesia. The objective of this study is to conduct an in vitro screening of 14 endophytic fungi isolated from Taxus sumatrana having antioxidative and α-glucosidase inhibitor activities. Each endophytic fungus was cultured for 7 days and the fungal mycelium and medium were extracted with methanol and ethyl acetate, respectively, to produce each extract. The antioxidative activity of each extract was tested by DPPH free radical scavenging activity and β-carotene bleaching assays, whereas antidiabetic activity was tested based on α-glucosidase inhibitor activity. The screening results showed that fungal mycelia of TSC 13 had the best α-glucosidase inhibitor activity and TSC 24 had the best antioxidative activity. Isolation of bioactive compounds from TSC 13 and TSC 24 is being conducted. This is the first report that endophytic fungi isolated from T. sumatrana exhibited anti α-glucosidase inhibitory and anti oxidative activities.
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