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Articles by S. Sugesh
Total Records ( 3 ) for S. Sugesh
  K. Feroz Khan , G. Sankar , K. Ramamoorthy and S. Sugesh
  An ornamental fish health issue has become an urgent requirement for the sustaining growth of marine ornamental industry. Salt marshes are extremely important medicinal plant for a variety of reasons. The present investigation, five different salt marsh plants were selected (Arthrocnemum indicum, Helitropium curassavicum, Ipomoea pes-caprae, Salicornia brachiata and Sesuvium portulacastrum) and crude extracts were prepared with three different solvents (aqueous, methanol and diethyl ether) and the crude extracts were tested against six fish bacterial pathogens isolated from marine aquarium (Escherichia coli, Aeromonas sp., Proteus sp., Pseudomonas aeroginosa, Pseudomonas fluorescence and Vibrio paraheamolyticus). The methanolic extracts of all salt marsh plants showed maximum zone of inhibition against Aeromonas sp. (14 mm) and minimum was observed in followed by moderate activity from diethyl ether extracts (3 to12 mm) and the aqueous extracts (2 to 10 mm) showed comparatively poor activity against all fish pathogens. The methanolic crude extracts of five salt marsh plants control the Aeromonas species. Methanolic extract of Helitropium curassavicum and Salicornia brachiata were estimated significant effective antibacterial activity against all tested bacterial strains. Helitropium curassavicum and Salicornia brachiata activity were reported relatively nearer to the tetracycline compound.
  S. Sugesh and P. Mayavu
  The marine invertebrates become one of hot spot for the lead of antimicrobial compounds. Two species of commercially available and edible bivalves (M. meretrix and M. casta) were assayed for antimicrobial activity against 10 bacterial pathogens and 6 fungal pathogens and its biochemical composition. The bivalves were extracted with three different solvent systems respectively methanol, ethanol and acetic acid. All the three extracts of both the species M. meretrix and M. casta showed highest antibacterial activities against S. aureus, E. coli, B. substillus, K. pneumonia, P. fleuroscence and V. cholera. In present investigation the methanolic extract of the two bivalve species of M. meretrix and M. casta was showed inhibition activities against all pathogenic fungal forms. The two bivalve extracts showed high amounts of protein content, which made the variation up to 160-180 μg mg-1 (wet weight). Both samples had low amount of carbohydrates 4.77-5.77 μg mg-1 and lipids 0.11-0.17 μg mg-1, respectively. The results of thin layer chromatography were revealed that presence of pink color spots it clearly indicates the presence of amino acid or peptides in bivalve’s samples. Presuming that the antimicrobial compounds were proteins or peptides. In SDS-PAGE on 12% gel, the crude proteins M. meretrix and M. casta showed 5-6 bands ranging from 45-223 kDa. They represent potential pharmacological leads perhaps possessing novel and uncharacterized mechanisms of action that might ultimately benefit the ongoing global search for clinically useful antimicrobial agents.
  P. Mayavu , S. Sugesh and V.J. Ravindran
  The present study was carried out on antimicrobial properties of seagrass species against biofilm forming bacteria’s from boat hull during the period April 2008 to March 2009. Seagrass species have a very potential groups were producing several secondary metabolites. The bioactive potential of two different seagrass species viz., Cymodocea serrulata and Syringodium isoetifolium occurring commonly along the Tuticorin coastal area were selected and preliminary effort has been made against the marine biofilm forming bacteria’s Pseudomonas aeruginosa, Bacillus cereus, Proteus vulgaris, P. mirabilis, E. coli, Listeria monocytogenes, Salmonella enteritidis, Staphylococus aureus and Vibrio paraheamolyticus, which also the human pathogens. The seagrasses of C. serrulata and S. isoetifolium were extracted with four different solvents such as ethanol, methanol, acetone and dichloroethane. Ethanol and methanol extracts of S. isoetifolium was inhibited the biofilm forming bacteria such as E. coli (14 mm), P. aeruginosa (8 mm) and V. paraheamolyticus (7 mm) and it showing Minimum activity against S. aureus (2 mm). The crude extract of ethanol and methanol of C. serrulata was inhibited the growth of all the 9 species of the biofilm forming microbes. The results of present study were concluded that seagrasses have potential bioactivity against marine biofilm forming microorganisms.
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