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Articles by S. Soleimanian-Zad
Total Records ( 2 ) for S. Soleimanian-Zad
  A.H. Mahdavi , H.R. Rahmani , N. Nili , A.H. Samie and S. Soleimanian-Zad
  The growth inhibitory effect of egg yolk antibody (IgY) powder for food application was investigated on Escherichia coli O78:K80, as a model and the major cause of associated septicemic disease in broiler chickens. Laying hen’s hyperimmunized with E. coli O78:K80 bacteria for a 12 weeks period and IgY was isolated by the water-dilution method. E. coli O78:K80 was incubated with IgY (50, 100 and 150 mg mL-1 specific and non-specific IgY powder) for 6 h during, which samples were taken at 2 h intervals. After the first immunization, the antibody specific activity in serum and egg yolk increased and became plateau on day 7 and 14, respectively. The protein concentration of serum and IgY powder did not change during the immunization period, but the ratio of gamma globulins to total serum protein and IgY purity (total IgY in protein) of IgY powder increased (p<0.05) and remained high throughout the experimental period. Specific IgY powder at the concentration of 150 mg mL-1 decreased bacterial proliferation by 1.18 log CFU mL-1 compared with the control group at 6 h of incubation period (p<0.01). Results of this study suggest that E. coli O78:K80 could have long term antigenic properties to induce an immune response in laying hens. The large-scale and long-term production of IgY, with high purity and high specific activity, can be attained by water dilution method. The inhibitory effect of specific IgY powder may encourage IgY powder to be applied as a feed additive to protect against E. coli O78:K80.
  M. Mirlohi , S. Soleimanian-Zad , M. Sheikh-Zeiondin and Hossein Fazeli
  Regarding the importance of the presence of intestinal lactobacilli and their population in infants, four different treatments were evaluated for Lactobacillus isolation efficiency via reduction in the growth of other groups of bacteria capable of growing on de-Man Rogosa Sharpe (MRS) medium from fecal samples of 11 Iranian infants. MRS-Vancomycin (1 mg L-1) was used as a base medium and application of lactic acid and aerobic incubation of inoculated plates were performed as selective factors. Each fecal sample was cultivated as duplicate on to the base medium with or without lactic acid to reduce the pH to 5.4 ±0.2. Half of the plates were incubated aerobically and the rest of them incubated under 10% CO2 concentration. Total count and Lactobacillus count of all samples were recorded according to the age differences of infants. The counts of false positive colonies were recorded with respect to their cell morphology and gram reaction in all treatments. Anaerobic incubation of lactic acid modified MRS-Vancomycin gave the most Lactobacillus percentage coverage, about 93% among the Lactobacillus positive samples. Using this treatment, the median Lactobacillus count yielded 8.29 log10 cfu g-1 in the younger and 5.70 log10 cfu g-1 in the elder group. It could be concluded that lactic acid might be a proper pH reducing agent when enumeration of lactobacilli from fecal samples is of interest.
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