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Articles by S. Razie
Total Records ( 2 ) for S. Razie
  S. Razie , M. Panahi , A. Ahangarpor , F. Rahim and G. Saki
  This study was designed to evaluate the ultrastructure of contra lateral testis tissue and measurement of Serum inhibin B following unilateral blunt testis trauma. Twenty pre-pubertal male wistar albino rats aged 3 weeks were divided into 4 equal groups that each containing five rats. Group I was the control group. Group II was used as a Sham group. Group III had right orchiectomy initially. Group IV was the trauma group in which the right testis was placed on a firm sterile surface and the metal rod weighting 100 g was drooped on to the testis from a height of 5.5 cm. Seven weeks after initial operation 3 mL blood samples were obtained from each rat to determine inhibin B levels and contra lateral orchiectomies were performed in all groups to microscopically investigate electron. Inhibin B levels decreased in groups 3 and 4. The difference between group 3 with groups 1 and 2 was significant (p = 0.003 and 0.02). Also the difference between group 4 with groups 1 and 2 was significant (p = 0.006 and 0.002) but the difference between group 3 and 4 was not significant (p = 0.08). In group III (orchiectomy) TEM showed a normal sperm morphology and normal disruption of different stages of the spermatogonial maturation. Debris and vacuolar changes were seen in sertoli cells. Morphology of leydig cells slightly modified and the dilated cisternae of the Smooth Endoplasmic Reticulum (SER) were observed in group IV( trauma) mitochondria with degenerated cristae and enlarged vacuole were observed.
  G. Saki , S. Razie and S. Amirpoor
  The present experimental study was designed to investigate to elucidative effect of forced swimming stress on female mice fertility with emphasis on oocyte number and their quality and ultimately their in vitro fertilization capacity. A total 30 adult NMRI (Noda Medical Research Institute) mice randomly divided into two equal groups (n = 15); 1) control and 2) experimental groups. The control group remained in their cages, but experimental group was submitted to forced swimming for 3 min in water at 32°C daily for 50 days. The NMRI female mice in two group of study who mention above were administered intra-peritoneally with 5 IU pregnant mare serum gonadotropin (PMG) for superovulation. This was followed 46-48 h later by the intraperitoneal administration of 5 IU Human Chorionic Gonadotropin (hCG). Mice were euthanized 12-14 h after HCG injection by cervical dislocation method and oocytes collected from fallopian tube. Then count and quality of oocyte were assessed. In order to evaluated of fertilization capacity, a pre-incubated capacitated sperm was gently added to the freshly collected ova of two groups of study and two-cell embryos was counted 24-26 h after completion of fertilization in vitro. The fertilization capacity of oocyte of exposed to forced swimming stress was significantly lower than control groups (p<0.05). The plasma level of FSH and LH was significantly increased in stress mice group (p<0.05). The data of this present study clearly showed that forced swimming stress do not alter number of oocyte extraction but quality of extracted oocyte significantly decrease in mice exposed to forced swimming stress.
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