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Articles by S. Rahighi
Total Records ( 1 ) for S. Rahighi
  A. GholamhoseinianNajar , S. Mansouri and S. Rahighi
  Myrtus communis L. (Myriaceae) has been reported to have antibacterial activity against Staphylococcus aureus. However, the mechanism of antibacterial activity of this medicinal herb is not so clear. The leaves of M. communis were extracted with chloroform, ethyl acetate and methanol successively and then Total Oligomeric Flavonoids (TOF) were obtained by applying precipitation method. Minimal Inhibitory Concentration (MIC) against Staphylococcus aureus (NTCT 8530) was determined by agar dilution method. Sub MIC (SIC) and 0.1 SIC of different extracts were used for the experiments. Bacteria grown with or without the extracts were lysed with lysophosphatin. The activities of catalase and superoxide dismutase (SOD) were measured by using a spectrophotometric method. Malonedialdehyde (MDA) was determined by thiobarbitoric acid method and the total antioxidant capacity was measured by colorimetry. Specific activity of SOD in bacteria treated by TOF, ethyl acetate, chloroform and methanol extracts at SIC were found to be 0.41, 0.42, 0.38 and 0.51, respectively, compared to 0.93 U mg-1 for the control sample, showing a significant decrease. Catalase specific activity of bacteria in media containing TOF, ethyl acetate, chloroform and methanol extracts were 50.55, 11.97, 46.66 and 46.67 U mg-1, respectively, which showed significant reduction compared to their controls (88.82 U mg-1, p<0.001). All four extracts of Myrtus communis leaves caused a decrease in lipid peroxidation as MDA formation and the total antioxidant activity in S. aureus (p<0.001). It is concluded that the antibacterial effect of Myrtus communis on S. aureus is partly due to induction of free radicals. Ethyl acetate extract showed the highest antibacterial effect comparing to all others. The effects of extracts on free radicals were dose dependent.
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