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Articles by S. Nagata
Total Records ( 2 ) for S. Nagata
  F.A. Bhuiyan , S. Nagata and K. Ohnishi
  Chitinase degrades chitin which comprises an important source of carbon and nitrogen in the marine environment. The aim of this study was to evaluate the population of chitinases in the marine sediments in southwest Japan. We collected marine sediments from eutrophic inner bay and offshore. Chitin-degrading bacteria were enriched from both sediments. Metagenomic DNA was isolated from the enriched chitin-degrading bacterial cell culture. At the same time, 25 chitin-degrading bacteria were isolated from the enriched culture. Partial fragments of chitinase genes were successfully amplified with degenerate primers designed for the glycoside hydrolase 18 family. We analyzed chitinase gene sequences of about 500 clones from metagenomic DNA prepared from chitin-degrading bacteria. Based on translated amino acid sequences, chitinases were grouped into five groups. Chitinases in groups II and III was most abundant and close to chitinase genes of several species of proteobacteria. On the other hand, chitinases in groups I, IV and V were unique and distinct from the known chitinases. These results indicate that the marine sediments used in this study contain diversity of chitinase genes.
  H. Sasaki , E. Iwata , A. Oshima , A. Ishida and S. Nagata
  We tried the isolation of halophilic bacteria from Asian desert dust falls in Japan and growth property of these bacteria and their molecular phylogeny were analyzed. Two Gram-positive bacteria designated as IMU-1 and IMU-2 were isolated from Asian desert dust. These two strains were adapted with 0-3 and 0-4 M NaCl under nutrient medium culture conditions, respectively, showing the properties of halotolerance. Under the Davis minimal medium culture condition, IMU-1 attained to the similar level of growth as that of nutrient medium culture and growth was observed at 0-2.5 M NaCl. On the other hand, IMU-2 showed the different growth as that of nutrient medium culture condition and growth was observed at 0-1.2 M NaCl. Phylogenetic analysis using 16S rRNA gene sequences revealed that IMU-1 and IMU-2 belong to Bacillus licheniformis and B. megaterium, respectively. It was first study about the isolation of B. licheniformis as the halophilic bacteria in Japan.
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