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Articles by S. Moshkelani
Total Records ( 2 ) for S. Moshkelani
  A. Sharifzadeh , A. Doosti and S. Moshkelani
  Leptin is a protein involved intricately in the growth and metabolism of animals and which plays an important role in the regulation of feed intake, energy metabolism, growth and reproduction of cattle. We used the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) technique to screen for DNA polymorphisms of the leptin gene in 112 Iranian Holstein cattle (Bos taurus) in Karaj Animal Breeding Center. PCR was carried out between exon 2 (intron 2). A strategy employing polymerase chain reaction was used to amplify a 422 bp from semen DNA. Digestion of polymerase chain reaction products with Sau3AI revealed two alleles: allele A was 390, 32 fragments and allele B was 303, 88, 32 (only 303 fragment visible on the gel). Three patterns were observed and frequencies were 60.71, 37.5 and 1.79% for AA, AB and BB, respectively. This polymorphism could be further evaluated for marker-assisted selection and developed PCR methodology would expedite screening for large numbers of animals required for such studies.
  S. Moshkelani , S. Rabiee and M. Javaheri-Koupaei
  Analysis of microsatellite markers as useful polymorphic genetic variations helps to characterize different animal species and breeds, moreover with develop molecular genetics techniques, beings identification and parentage testing is possible using a lot of molecular markers. So in the present study, the researchers examine the 14 genetic markers AHT5, AHT4, ASB23, ASB17, ASB2, VHL20, CA425, HMS7, HMS6, HMS3, HMS1, HTG4, HTG10 and LEX3 of Iranian Arab horses and use they in identity testing it species in Iran. To achieve this goal, DNA was extracted from blood samples collected from 13 families of Iranian Arab strain and then the multiplex polymerase chain reaction was used for amplification of fourteen markers with the specific primers and the PCR products were resolved on a non-denaturing 10% polyacrylamide gel by electrophoresis. The PCR products also remaining obtainable with formamide and electrophoresis was carried out on an ABI PRISM 3100 genetic analyzer using the recommended protocols. The average of heterozygocity was 0.656 and the expected of heterozygocity at this population was 0.697. Consequently, it seems that these fourteen markers can be used as an applicable marker for identifying Arabian horse.
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