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Articles by S. Mansouri
Total Records ( 3 ) for S. Mansouri
  A. GholamhoseinianNajar , S. Mansouri and S. Rahighi
  Myrtus communis L. (Myriaceae) has been reported to have antibacterial activity against Staphylococcus aureus. However, the mechanism of antibacterial activity of this medicinal herb is not so clear. The leaves of M. communis were extracted with chloroform, ethyl acetate and methanol successively and then Total Oligomeric Flavonoids (TOF) were obtained by applying precipitation method. Minimal Inhibitory Concentration (MIC) against Staphylococcus aureus (NTCT 8530) was determined by agar dilution method. Sub MIC (SIC) and 0.1 SIC of different extracts were used for the experiments. Bacteria grown with or without the extracts were lysed with lysophosphatin. The activities of catalase and superoxide dismutase (SOD) were measured by using a spectrophotometric method. Malonedialdehyde (MDA) was determined by thiobarbitoric acid method and the total antioxidant capacity was measured by colorimetry. Specific activity of SOD in bacteria treated by TOF, ethyl acetate, chloroform and methanol extracts at SIC were found to be 0.41, 0.42, 0.38 and 0.51, respectively, compared to 0.93 U mg-1 for the control sample, showing a significant decrease. Catalase specific activity of bacteria in media containing TOF, ethyl acetate, chloroform and methanol extracts were 50.55, 11.97, 46.66 and 46.67 U mg-1, respectively, which showed significant reduction compared to their controls (88.82 U mg-1, p<0.001). All four extracts of Myrtus communis leaves caused a decrease in lipid peroxidation as MDA formation and the total antioxidant activity in S. aureus (p<0.001). It is concluded that the antibacterial effect of Myrtus communis on S. aureus is partly due to induction of free radicals. Ethyl acetate extract showed the highest antibacterial effect comparing to all others. The effects of extracts on free radicals were dose dependent.
  F. Aram , G.H. Shahidi Bonjar , M.J. Mahdavi , S. Mansouri , P. Rashid Farrokhi , N. Bana Hosein Pour , M. Shekari , A. Ghasemi and S. Aghighi
  Streptomyces sp. are mostly soil-inhabitants and several species are responsible for important diseases in plants, S. scabies causal agent of Common Scab disease of potato and human S. somaliensis causal agent of Mycetoma. Methanolic extracts of leaves of Myrthus communis and seeds of Terminalia chebula, which had documented uses in Iranian herbal-medicine, were tested for anti-Streptomyces activity against S. scabies. For bioassays, concentration of 50 mg mL-1 of methanolic extracts prepared in dimethyl sulfoxide: methanol (v/v, 1/1) and tested in well diffusion method. Inhibitory zones (IZ) in contrast to controls were measured five days after inoculation at 29°C. Both plants showed high biological activity against S. scabies. Future goals include bioassays on human and further plant pathogenic Streptomycetes.
  S. Mansouri , E. Ghasami and N. Shahabi Najad
  The objective of present study was to determine reliable data on vaginal carriage, serotype distribution and antibacterial susceptibility of Streptococcus agalactiae, Group B streptococcus (GBS) in pregnant women in southeast of Iran. Vaginal swab cultures for GBS were obtained from 602 pregnant women at childbirth. Susceptibility of the isolates to penicillin, ampicillin, clindamycin and erythromycin were determined by standard agar dilution method. Isolates were classified according to their capsular polysaccharide types. GBS was isolated from 55 pregnant women (9.1%). All isolates were sensitive to penicillin and ampicillin [Minimum Inhibitory concentration (MIC) range of 0.03-16 μg mL-1 and ≤ 0.03-1 μg mL-1, respectively). Erythromycin and clindamycin resistance were seen in 10.9% (MIC range ≤ 0.03-16 μg mL-1) and 25.4% (MIC range ≤ 0.03-32 μg mL-1) of the isolates, respectively. Serotype III (41.8%), Ib (25.45%) and II were the most frequently isolated serotypes (14.54%). Group IV was not detected and 14.54% of the isolates were non type-able. No correlation was found between GBS colonization and demographic factors of age, parity, history of abortion or ruptured membrane and vaginal signs or symptoms. In conclusion the rate of GBS colonization is low in this area, but serotype III, which is mostly involved in invasive disease is the predominant serotype. Routine maternal screening should be performed to prevent group B streptococcal disease in neonates in this district. Susceptibility to the isolates to clindamycin and erythromycin should be checked in the penicillin-allergic patients, to avoid treatment failure.
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