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Articles by S. Lumyong
Total Records ( 4 ) for S. Lumyong
  S. Techaoei , S. Lumyong , W. Prathumpai , D. Santiarwarn and P. Leelapornpisid
  The aim of this research was to study isolation, characterization and stability of biosurfactant-producing bacteria. One hundred and ninety-seven bacterial strains (69 from hot springs and 128 from garage sites) were isolated from Northern Thailand and screened for biosurfactant production, determined for its activities by oil displacement area measurement, emulsification test and surface tension measurement on parafilm. Twenty-five strains of bacteria from garages site showed a promising biosurfactant production. Strain SCMU106 that presented the highest values of oil displacement area (143.20 cm2), emulsification index (60%) and droplet formation on parafilm surface (8.0 mm) was identified as Pseudomonas aeruginosa by 16S rDNA sequence analysis. Combination of 1% glucose plus 0.1% corn oil and 0.5% (NH4)2H2PO4, as carbon and nitrogen source, respectively was the optimal biosurfactant producing condition. The effects of pH, salinity and temperature on the produced biosurfactant were also evaluated. The produced biosurfactant was non-cytotoxic and stable to high temperature, neutral to alkaline pH (pH 6-10) and moderate concentration to electrolyte (up to 8% NaCl).
  P. Nandakwang , S. Elliott , S. Youpensuk , B. Dell , N. Teaumroong and S. Lumyong
  Arbuscular Mycorrhizal (AM) status of native plants in the tropical forest of northern Thailand was surveyed. Twenty four framework tree species, used to forest restoration were examined at 3 sites: FORRU=s research tree Nursery (FN), Forest Restoration plot (FR) and Natural Forest (NF). Eleven dominant herb species were examined at 2 sites: Degraded Watershed (DW) and Forest Soil extraction area (FS). Rhizosphere soil samples were collected and AM fungal spores were counted and identified morphologically. Most plant species were intensively colonized by AM fungi except Cyperus cyperoides. Twenty four AM species were identified: Glomus (15 species), Acaulospora (6 species) and Scutellospora (3 species). Glomus rubiforme was the dominant species. Spore density varied from 16.1 to 97.4 per 100 g soil (averaged 59.7). Spore number at DW and FS were 129 and 479 spores, respectively, with species richness of 6 and 8, respectively. Spore number at FN, FR and NF were 1,152, 2,337 and 1,376 spores, respectively, with species richness of 17, 21 and 15, respectively. The AM diversity was lower in the sites dominated by herbs than in sites examined for trees. In the deforested sites, reduced plant diversity was related with reduced mycorrhizal diversity. In contrast, the trial plot had the highest AM fungal community. Therefore, the forest restoration techniques allow tree species grown in nursery to become AM associated. The association is still maintained after planting out trees in restored area.
  P. Nandakwang , S. Elliott , S. Youpensuk and S. Lumyong
  Castanopsis acuminatissima is a native tree used to restore forest in Thailand. To accelerate seedling growth experiments were carried out to determine the efficacy of applying to C. acuminatissima. Arbuscular Mycorrhizal (AM) fungi, produced on sorghum, were used as inoculum to investigate the symbiosis on seedlings. The effects of AM inoculation (Acaulospora elegans, Glomus etunicatum, Glomus mosseae) together with phosphate fertilization (KH2PO4) on seedlings in a P-deficient soil were studied under greenhouse conditions. Increasing P-application rates greatly enhanced seedling growth (maximum at 250 mg kg-1 soil). Growth was most rapid with G. etunicatum-colonized plants with P application (40.8 cm), whereas much lower height was found with non-AM plants without P added (14.4 cm). The mycorrhizal effective for C. acuminatissima in previous experiments were confirmed by growing seedlings in a forest soil with slow-release fertilizer (NPK) and combined with AM species under nursery performance conditions. Plant height was significantly enhanced by fertilizer but not by fungi. The greatest height was found in non-AM plants with fertilization (14.5 cm), whereas lower height was found for non-AM plants with no fertilizer added (10.9 cm). AM inoculation greatly enhanced seedling growth in P-deficient soil more than in forest soil due to differences in abilities of AM species to establish a symbiosis. Therefore, in sapling production, the soil properties and level of fertilization should be evaluated keeping secondary effects caused by changed mycorrhizal association.
  W. Nuangmek , E.H.C. McKenzie and S. Lumyong
  Screening of endophytic fungi which antagonize Colletotrichum musae, the cause of anthracnose disease, was carried out. The in vitro screening was studied by dual culture method. The inhibition due to fast competitive growth of Cordana sp. (KPP-3) and the antibiotic producing endophyte; Nodulisporium sp., reached a high percentage (90% C. musae inhibition). Spore germination assay showed 91% C. musae germination, while only 0.63 and 1.88% germinated in the conidial suspension of Cordana sp. and Nodulisporium sp., respectively. There was a significant difference between the Disease Severity Index (DSI) of banana fruits treated with Cordana sp. and those treated with Nodulisporium sp. The results presented in this research highlight the possibility of using endophytic fungi as biological control agents for anthracnose disease of banana.
 
 
 
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