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Articles by S. Khairani-Bejo
Total Records ( 9 ) for S. Khairani-Bejo
  O. Shahaza , S. Khairani-Bejo , Z. Zunita and A.R. Bahaman
  The Rose Bengal Plate Test (RBPT) antigens from Brucella melitensis local isolates (in-house RBPT) were prepared and compared with RBPT antigen for Brucellosis in sheep and goats prepared by Veterinary Laboratory Agency, UK. Eight hundred fifty-six sera samples, of which were collected from goats were examined with the RBPT and Complement Fixation Test (CFT). The RBPT and CFT results showed that the in-house RBPT antigen was superior to the commercial prepared RBPT antigen (VLA, UK). Out of 856 sera analyzed by in-house RBPT, commercial RBPT and CFT, 30.84, 26.40 and 31.65% were found to be Brucella positive, respectively. The sensitivity calculated for the in-house RBPT compared with CFT was 85.24% whilst that of commercial RBPT was 78.59%. Therefore, it was conclude that in-house RBPT antigens could be prepared and used for epidemiological surveillance of Caprine brucellosis in Malaysia.
  Maged Ahmed AL-Garadi , S. Khairani-Bejo , Z. Zunita and A.R. Omar
  Isolation of Brucella melitensis is the standard gold of identification and confirmation of animal brucellosis. However in Malaysia, Brucella sp., infection of goat was increasing recently and there is no evidence for diagnosis of the serover of Brucella sp., that cause the disease in goat population except the detection of serological methods. Isolation and identification of Brucella melitensis have been done by bacteriological methods in addition to conventional Polymerase Chain Reaction (PCR) and Real-time PCR for detection of Brucella melitensis from samples collected from vaginal swabs on suspected farm. In conclusion, four isolate have been got out of 300 vaginal samples and all isolate is belong to Brucella melitensis server 1. The Real-time PCR is the easy and save method for confirmation of brucellosis in goats population.
  S. Ahmad , M. Hair-Bejo , Z. Zunita and S. Khairani-Bejo
  Salmonella enteritidis (SE) has always been related to subclinical infection in the chickens infected after 2 weeks of hatching. However, few pathogenic phage types were proven for their ability to manifest systemic infection and cause the organism to be shed into the surrounding environment. It was the objective of the study to determine the pathogenicity of SE Phage Type (PT) 1 in Specific-Pathogen-Free (SPF) chickens. About 93, 21 day old SPF chickens where divided into 3 groups namely the Control, SE and Mortality groups. The chickens were raised separately in caging system and given free access to antibiotic-free ration and water. The SE and Mortality groups were inoculated orally (1.0 mL) with SE PT 1 (1x108 cfu mL-1). The chickens in the SE and Control groups were sacrificed at various intervals throughout the trial. Samples were collected for bacterial isolation and histological examination. The mortality percentage of the chickens in the Mortality group was recorded. The study showed that no mortality was recorded throughout the trial in the mortality as well as the SE group. Body weight was lower in the SE group when compared to the Control group throughout the trial except at days 2, 3 and 5 post inoculation (pi) reaching its peak at day 14 pi when the SE group body weight was 26% lower than the controls. Clinical signs observed in the SE and Mortality group were represented by diarrhoea, inappetance, ruffled feather and stunted chickens while no abnormal clinical signs where recorded in the Control group. Grossly mild airsacculitis, mild peritonitis and hepatic congestion where recorded in the SE group at day 2 pi until day 5 pi while no gross lesions where recorded in the Control group. SE was first isolated in the caecum (66%) at 12 h pi. At day 1 pi SE was isolated from the caecum and spleen (33%) whilst at day 2, SE was isolated from the caecum (100%) and caecal tonsil (66%). No SE was isolated from the cloacal swabs throughout the trial. The villi height was generally lower in the SE group when compared to the Controls, however it was significantly lower (p<0.05) in the duodenum at 12 h, days 1, 3, 5, 10, 14 and 21 pi; in the jejunum at 6 h, days 2, 14 and 21 pi while in the ileum at days 1, 3 and 5 pi. The crypts depth measurement was fluctuating however it ended up by being higher in the SE group, nevertheless it was significantly lower (p<0.05) in the SE group when compared to the Control group in the duodenum at 6 h and day 14 pi in the jejunum at day 10 pi; in the ileum at 12 h pi. Histopathological changes recorded included hepatitis, congestion and focal areas of necrosis; splenitis, congestion and oedema in the adenoid sheathed arteries; congestion and areas of necrosis in the lymphoi follicles of the bursa of Fabricius; enteritis, congestion and sloughing of necrotic enterocytes in the intestinal villi with presence of bacterial clusters in the villi surface and intestinal lumen. SE rods present in the caecal tonsils were seen to be engulfed by macrophages at days 1 and 2 pi, necrosis of the enterocytes on the villi surface and infiltration of the bacteria was recorded at day 2 pi while at days 5 pi the bacteria multiplication were seen and often located upon the M-like M cells however, no actual engulfment was recorded.
  Maged Ahmed AL-Garadi , S. Khairani-Bejo , Z. Zunita and A.R. Omar
  PCR assays have been shown to be a promising option for the diagnosis of brucellosis. However, there is no study conducted in Malaysia to identify the brucellosis in goat’s population. In this study three whole blood samples and sera were collected from goat’s farm in Kedah state Malaysia which was suspected to have brucellosis. Serological and molecular detection of brucellosis have been done including RBPT, CFT, conventional PCR and Real time. The evaluation of each test have been discussed rather than the sensitivity and specificity of the each test which can be used in Malaysia national eradication programs. In conclusion, the combination between the serological test and molecular technique specially real time PCR depend on IS711 region in hypothetical protein is promising and can be reduced to false positive result which can cause heavy economical loss during controlling programs.
  Y.O. Abdinasir , F.F.A. Jesse , A.A. Saharee , S. Jasni , S. Khairani-Bejo and A.W. Haron
  Acute Phase Proteins (APP) are blood proteins that contribute to restoring homeostasis and limiting microbial growth in an antibody-independent manner in animals subjected to infection, inflammation, surgical trauma or stress. There are still lack of knowledge of acute phase protein profiles in mice associated with infection of Corynebacterium pseudotuberculosis and its exotoxin Phospholipase D (PLD). In this study, serum concentrations of three different positive Acute Phase Proteins (APPs) are studied; Serum Amyloid A (SAA), Haptoglobin (Hp) and α1 Acid Glycoprotein (AGP). This study was conducted to acquire a better way of understanding the pathophysiology response of Corynebacterium pseudotuberculosis and its exotoxin in mice model. A total of 48 mice, 2-3 weeks of old both sexes were enrolled and equally divided into three groups; namely control, whole cell and exotoxin groups. Mice of whole cell groups were exposed intraperitoneally to 1 mL of the inoculums containing 109 Colony-Forming Unit (CFU) mL of live C. pseudotuberculosis. Exotoxin group were infected intraperitoneally with a single dose of exotoxin (PLD) extracted from C. pseudotuberculosis. While control group were exposed intraperitoneally to 1 mL of Phosphate-Buffered Saline (PBS), pH 7. Blood samples were collected by cardiac puncture for acute phase protein analysis. All APPs were quantified by commercially available ELISA methods and AGP was assessed by highly sensitive clourometric assay. The results revealed that there were statistically significant differences (p<0.05) between APPs concentrations throughout the experimental period in groups of mice induced with whole cell and exotoxin of C. pseudotuberculosis compared to control groups. The concentrations of Hp and SAA were significantly induced after infection of C. psedotuberculosis with mean maximum levels from days 1-4 of post infection whereas the AGP concentration was significantly induced after 3rd day of post infection. About >70 fold increase was observed in Hp concentrations after experimentally induced whole cell and single intraperitoneal dose of exotoxin whereas SAA and AGP increased <15 fold. No significant differences (p>0.05) were observed in acute phase proteins profiles between whole cell and exotoxin groups. Therefore, the results of this study indicated that acute phase proteins can be used as potential biomarkers for assessing caseous lymphadenitis.
  Y.O. Abdinasir , F.F.A. Jesse , A.A. Saharee , S. Jasni , S. Khairani-Bejo and A.W. Haron
  The purpose of the study was to describe the systematic infections of caseous lymphadenitis in mice with special reference to clinical manifestation and pathogenesis. In this, 104 apparently healthy mice, 2-3 weeks of old were selected and divided into 3 groups, namely control, whole cell and exotoxin groups. Mice of whole groups were exposed intraperitoneally to 1 mL of the inoculums containing 109 Colony-Forming Unit (CFU)/mL of live C. pseudotuberculosis. Exotoxin group were infected intraperitoneally with a single dose of exotoxin extracted from C. pseudotuberculosis. Control group were exposed intraperitoneally to 1 mL of phosphate-buffered saline. Groups of exotoxin and whole cell challenged showed prominent clinical signs that were characteristic of CLA which included depression, anorexia, submandibular oedema, yellowish and bloody diarrhea, ruffled caot, eye discharges, poor general condition and occasionally partial tremor of hindquarters. In necropsy, visceral abscess was the condition recorded in experimentally infected mice. There were also congestion and hemorrhages in the lungs, liver, spleen, kidney and intestine. Microscopically, there were lesions in the form of tuberculous granuloma (caseating tubercule), presence of giant cells, infiltration of neutrophils and macrophages, degeneration, vacuolation (necrosis), hemorrhage and formation of microabscesses. The results of this study indicated that manipulation of the organism and its extracted exotoxin (PLD) in experimenatal animals (mice model) led to clinical signs and pathological alterations that were similar to those conducted on small ruminant research of experimentally nature.
  I.M. Ahmed , S. Khairani-Bejo , L. Hassan , A.R. Bahaman and A.R. Omar
  The potential diagnostic ability of Recombinant Outer Membrane Proteins (rOMPs), a combination of equal concentrations of rOMP25, rOMP28 and rOMP31of Brucella melitensis was investigated using Enzyme-Linked Immunosorbent Assay (ELISA) to differentiate the False Positive Serological Reactions (FPSR) in the serological diagnosis of caprine brucellosis. The rOMPs was tested using sera from three groups of goats with known Brucella exposure status which represent, naturally B. melitensis infected goats (infected), Brucella free goats (non-infected) and goats vaccinated with B. melitensis Rev. 1 vaccine strain (vaccinated). Additionally, all the sera were tested using the common serological tests which are Rose Bengal Plate Test (RBPT), BRUCELISA-400SG and Complement Fixation Test (CFT). When testing infected and non infected groups, the rOMPs I-ELISA recorded 94.44% (34/36) sensitivity and 100% (36/36) specificity and this almost agreed with the results obtained from testing the same serum samples using RBPT, BRUCELISA-400SG and CFT. However, when goats vaccinated with B. melitensis Rev. 1 vaccine strain were tested by the common serological tests, RBPT, BRUCELISA-400SG and CFT they wrongly recorded positive results for all the tested serum samples (26/26). While the developed rOMPs I-ELISA was able to differentiate the vaccinated from infected animals with 94.44 sensitivity and 84.62% specificity. The potential diagnostic ability of rOMPs would be of great importance as serologic marker to minimize the FPSR in eradication programs of caprine brucellosis.
  P.H. Bamaiyi , L. Hassan , S. Khairani-Bejo , A. Adzhar and M. Ramlan
  A study was conducted to detect Brucellosis in pigs using the Brucella abortus Complement Fixation Test (CFT). Out of a total sample of 552 pigs 3 (0.54%) were positive for Brucellosis. This result has epidemiological implications for the transmission of brucellosis in Malaysia and beyond due to the interaction of pigs with other animals and the human population.
  S.N. Mohamed-Hassan , A.R. Bahaman , A.R. Mutalib and S. Khairani-Bejo
  Rats are considered as one of the most important sources of leptospirosis as they are present in abundance in many environments. They caused significant economic losses and served as reservoirs for many zoonotic diseases. One of the diseases is leptospirosis which is considered a re-emerging disease in Malaysia. However, knowledge of the epizootic of leptospirosis and leptospiral serovars associated with rats is lacking. The objectives of the study therefore were to determine the distribution of rat’s species and their carrier status. In Malaysia, R. tiomanicus was found to be the dominant species found in Malaysian environments and it constitutes 86.0% (420 out of 488 rats) of the rats caught in different types of localities; National Service Training Camps (Kelantan, Terengganu, Malacca and Selangor), oil palm estates (Terengganu and Malacca), Royal Belum Rainforest (Perak), Suburban areas (Kelantan and Perak) and PULAPOL (Negeri Sembilan). Sixty leptospiral isolates (12.3%) were successfully cultured from the kidneys of the rats caught. Polymerase Chain Reaction (PCR) assay revealed 42 (8.6%) of the isolates were pathogenic as disclosed by the 16S primers. Majority of the pathogenic leptospires were isolated from rats caught in the National Service Training Camps (NSTC). The high rate in NSTC posed a major threat to the trainees as they were frequently involved in outdoor activities and exposed to infected environment. Therefore, control of rat population is crucial in minimizing the risk of transmitting leptospirosis to human.
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