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Articles by S. Karthigayan
Total Records ( 4 ) for S. Karthigayan
  R. Karthikeyan , S. Karthigayan , M. Sri Balasubashini , S. Vijayalakshmi , S.T. Somasundaram and T. Balasubramanian
  The present study was carried out to assess the antitumor effect of venom from snake, Hydrophis spiralis on the Ehrlich Ascites Carcinoma (EAC). Four groups of albino Swiss mice were treated with three doses of the sub lethal dose of venom, viz., 0.418, 4.18 and 6.27 μg kg-1 body weight (intraperitoneal injection) along with the standard drug 5 flurouracil (20 mg kg-1 b.w.). The biochemical analysis and rest was left to calculate the mean survival time. In EAC bearing mice, mean life span tumor volume, hemoglobin, red blood cell and lymphocytes were significantly decreased when compared to the normal animals. Whereas, body weight, neutrophils and viable tumor cell count was increased in the EAC bearing mice. These changes were brought back to near normal levels in different treatment groups. The macromolecule concentration of peritoneal cells, such as, DNA, RNA and protein, were altered in the EAC bearing mice and observed to be near normal in the treatment groups. The caspase 3 activity was significantly increased in the peritoneal cells of the treatment groups when compared to the EAC bearing mice. The role of apoptotic cascade in EAC cell death was confirmed by the DNA fragmentation on agarose gel. Apart from the antitumor effect, snake venom reduced the tumor burden on the liver and altered the changes in the activities of alanine transaminase (ALT), aspartate transaminase (AST) and alkaline phosphatase (ALP). Hence the venom from H. spiralis has a potential antitumor effect on the EAC bearing mice.
  R. Karthikeyan , S. Karthigayan , M. Sri Balasubashini , S. Vijayalakshmi , S.T. Somasundaram and T. Balasubramanian
  The anti-tumor activity of the sea snake venom (Hydrophis spiralis) was evaluated against Ehrlich’s Ascites Carcinoma (EAC) in Swiss albino mice and HeLa and Hep2 tumor cell cultures. Among the different dose tested 4.18 μg mL-1 at 24 h was found to effectively inhibit cancer cell proliferation. The same dose on EAC-bearing mice by i.p. injection significantly reduced the tumor growth and was demonstrated by increased life span of the mice by 182.81%.
  S. Karthigayan , M. Sri Balasubashini , M. Sengottuvelan , T. Balasubramanian and S.T. Somasundaram
  The present study was carried out to evaluate the anticancer potential of salivary gland extracts of Octopus ageina on in vitro and in vivo cancer models. LD50 values of both anterior (AGE) and posterior gland extract (PGE) were analyzed using Swiss Albino mice. Different concentration of sublethal dose was subjected to hemolytic assay using 1% human erythrocytes. Since PGE did not show any hemolysis at concentration below 400 μg, it was chosen for the anticancer studies. Preliminary anticancer effect was tested on COLO 205 cells at three different concentrations, viz., 50, 75 and 100 μg mL–1 medium. Among the above three doses, 100 μg mL–1 medium was found to inhibit cell growth effectively after 24 h. The same effective dose was used to confirm the anticancer potential of PGE against 1.2-dimethylhydrazine (DMH) induced colon cancer in male Albino Wistar rats. After 30 weeks of the experimental period PGE showed promising effects by reduced tumor incidence and mean tumor size. The β-glucuronidase (intestine and colon) and mucinase (Colon and fecal contents) activity were significantly reduced after treatment with PGE when compared to the DMH treated rats. The above anticancer effect of PGE was further confirmed by the histopathological changes of the colon. Thus, PGE proves itself to have potent anticancer substance and further studies are warranted to isolate and identify the active component.
  R. Karthikeyan , S. Karthigayan , M. Sri Balasubashini , S. Vijayalakshmi and T. Balasubramanian
  The venom of the Lapemis curtus was tested for its ability to induce histopathological changes in mice intraperitoneal injection of the venom (LD50 of 0.65 mg kg-1), by light microscopic examination of some organs (liver, kidney and spleen). L. curtus venom induces changes including necrosis and edematous appearance with cellular infiltration and vacuolation. The injury of kidneys includes significant changes of the glomerular apparatus. Venom treated mice liver shows congestion, micro vesicular fatty changes and infiltration of inflammatory cells around the portal vein. Where as, spleen showed hemorrhage, congested and inflammation were observed. Areas of hemorrhage, vascular congestion and cloudy swelling in renal tubules were observed in the kidney. No myoglobinuria was noted in any group of animals. The crude venom was also administered intraperitoneally into the experimental animals and tissue samples were taken at several time intervals. The venom of the sea snake L. curtus, was tested for its ability to induce myonecrosis changes in albino mice. Induction of myonecrosis was demonstrated by their ability to release Creatine Kinase (CK) from damaged muscle fibers and direct histopathological examination of the injected muscles (i.m.). Crude venom exhibits intense myonecrosis characterized by the changes including, necrosis and edematous appearance with cellular infiltrate, vacuolation and degenerated muscle cells with delta lesions and heavy edema in between the cells.
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