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| Articles
by
S. Kadzimin |
Total Records (
1 ) for
S. Kadzimin |
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A.E. De Silva
,
M.A. Kadir
,
M.A. Aziz
and
S. Kadzimin
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The induction of callus from Meristemic
Globular Bodies (MGB) of two pineapple cultivars, namely Moris and Josapine,
under six concentration levels of auxin NAA and six concentration levels
of 2,4-D in Murashige and Skoog solid media, was investigated. 2,4-D auxin
treatments failed to induce calli in both cultivars. However, 53.71, 75.19
and 85.93 μM levels of auxin NAA caused calli induction in Moris
while levels 32.22, 53.71 and 75.19 μM also induced calli Josapine.
The percentage of MGB calli formation increased with increasing time of
culturing. At 6 weeks of culturing, 83% of Moris MGB explants formed calli
on 85.93 μM NAA, while 50% of Josapine MGB explants formed calli
on 53.71 μM NAA. Calli cultures have been an essential tool in the
in vitro selection of desirable plants under manipulated conditions
and from in vitro mutations via somaclonal variation. More importantly,
calli are increasingly used for the application of cellular level genetic
modification techniques such as the Agrobacterium-mediated transformation,
particle bombardment and protoplast isolation and fusion. In this study,
auxin NAA successfully initiated and proliferated calli in Moris and Josapine
globular meristemic cultures. |
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