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Articles by S. Jayakumar
Total Records ( 2 ) for S. Jayakumar
  G. Gandhimathi and S. Jayakumar
  In the emerging scenario, Multicast conferencing plays an important role in e-education and e-business The multi conferencing systems have several shortcomings such as the conventional select-and-forward conference bridges gives inconsistent speech quality, due to full mesh and multicast architectures needs a large bandwidth at the endpoints. For a large scale voice conferencing through IP, now-a-days a tandem free architecture is coming up to reduce the bandwidth since it uses the compressed Low bit rate (Narrow Band) speech. The speech quality is to be increased by employing Artificial Bandwidth Extension (BWE) algorithm provided by Application Software Provider (ASP) at the endpoints. In this study, a new approach, the SaaS (Software as a Service) of cloud computing service is introduced to import Artificial BWE algorithm in to the cloud and the performance of SaaS over ASP is analyzed.
  S. Jayakumar and Sarvjeet Kaur
  Bacillus thuringiensis (Bt) has been used as biopesticide sprays due to its insecticidal specificity but precipitation loss is a limitation. Bt isolates, naturally occurring on crop phylloplanes, have better on-plant persistence. Bt isolates have been isolated and characterized from phylloplanes of leguminous crops. Bt isolates, which showed presence of highly conserved 16S-23S rRNA internal transcribed spacer region, were screened by PCR for cry gene families. The cry1 gene family was found to be most abundant, followed by cry2 gene family, while none of isolates showed presence of cry3, 4, 7 and 8 gene families. Bt isolates were further screened for presence of specific genes of cry1 gene family. Four isolates-SK-222, SK-223, SK-229 and SK-232, were found to have cry1Aa, cry1Ab, cry1Ac and cry1D genes. Isolate SK-222 was found to contain maximum types of genes followed by SK-223, SK-229 and SK-232. Protein profiles of isolates by SDS-PAGE showed 130 kDa band corresponding to cry1 protein. Isolate SK-223 was most toxic followed by SK-222, SK-229 and SK-232 towards diamondback moth (Plutella xylostella) by leaf dip bioassay. Isolates SK-222 and SK-223 were significantly more toxic than Bt subsp. kurstaki (HD1). These isolates have potential of development into biopesticides. Full length cry 1Ab gene was amplified by PCR with specially designed primers from isolate SK-222, cloned and sequenced (GenBank accession No. DQ023297). Sequence analysis showed it to be identical to cry1Ab18 gene. (, GenBank accession No. AY319967) isolated previously from a Bt isolate recovered from soil of Ladakh region in this laboratory.
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