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Articles by S. Hasani
Total Records ( 5 ) for S. Hasani
  S. Arbabi , T. Ghoorchi and S. Hasani
  The main objective of this study was to determine the effects of organic acid-based additives on the fermentation and delayed ensiling of corn silage. Prolonged exposure to air can adversely affect the silage fermentation process. To investigate a possible method to overcome this problem, we found that when a buffered propionic acid-based additive, is applied to chopped, whole-plant corn exposed to air before ensiling, it will affect the subsequent fermentation. Chopped whole plant corn mixed with 4 different additives consist of propionic acid in addition to control treatment without any additive. Additives were: (1) propionic acid, (2) propionic acid (85%)+formic acid (15%), (3) calcium propionate and (4) propionic acid (80%)+formic acid (15%)+ammonia (5%). The 10 g kg1 dry matter of each additives mixed with chopped corn forage in 3 different times (0 (immediately), 24, 48 h). Silages were assessed by the method of appearance evaluation and DM, pH evaluation. CP, NDF, ADF, TVFA, WSC, so that, the aerobic stability and DM degradation of each treatment were determined after 60 days. Silages that exposed to air for 24 h, before ensiling had better appearance quality than two other delaying time (0 and 48 h) in Filg's method and whole additives in this experiment had good effects on appearance quality in comparison with control group. All of silages containing buffered propionic acid-based additive, in method of DM, pH evaluation, were good and very good. These silages had lower (p<0.05) pH than control ones. Amount of dry matter of control silage which ensiled immediately was lower than other treated silages. NDF in control silages (without additive) was more than that in treated silages and amount of CP and TVFA increased with addition of additives especially those containing propionic acid (85%)+formic acid (15%) (p<0.05). WSC in control silage that wilted 48 h before ensiling was more than other samples (p<0.05). Buffered propionic acid-based additives increased aerobic stability in treated silages in comparison with untreated ones. Degradation of DM (in situ) for untreated silages that delayed ensiled was lower. Application of the additive containing propionic acid (80%)+ formic acid (15%)+ammonia (5%) resulted in highest degradation of dry matter among experimental additives. Generally, treatment with propionic acid-based additives prevented a decrease in DM degradation (in vitro).
  S. Arbabi , T. Ghoorchi and S. Hasani
  In order to study the effect of delayed ensiling and application of some organic acid-based additives on fermentation of corn silage, chopped whole plant corn mixed with 4 different additives consist propionic acid in addition to control treatment without any additive. Additives were: 1-Propionic acid 2-Propionic acid (85%) + Formic acid (15%) 3-Calcium propionate 4-Propionic acid (80%) + Formic acid (15%) + Ammonia (5%). 10 g kg-1 dry matter of each additives mixed with chopped corn forage in 2 different times (0.48 h). Silages were assessed using method of appearance evaluation and method of DM, pH evaluation CP, NDF, ADF, TVFA, WSC, aerobic stability and DM degradation each of treatments were determined after 60 days. Silages that exposed to air 48 h, before ensiling had better appearance quality in Filg`s method and whole additives in this experiment had good effect on appearance quality compared with control group. All of silages containing buffered propionic acid-based additive, in the method DM, pH evaluation, were good and very good. These silages had lower pH than control (p<0.05). Amount of dry matter of control silage which ensiled immediately was lower than other treated silages. NDF in the silages (with Propionic acid (85%) + Formic acid (15%)) was lower than the other silages and content of CP and TVFA increased with addition of the additives especially additive containing Propionic acid (85%) + Formic acid (15%) (p<0.05). WSC in treated silage with additive containing propionic acid (85%) + Formic acid (15%) that wilted 48 h before ensiling was more than other samples (p<0.05). Buffered propionic acid-based additives caused to increase aerobic stability in treated silages compared with untreated silages. Degradation of DM (in situ) in untreated silages that delayed ensiled was lower. Application of the additive containing propionic acid (80%) + Formic acid (15%) +Ammonia (5%) resulted in highest degradation dry matter among experimental additives. Generally, treating with propionic acid-based additives prevented degradation DM decrease (in vitro).
  S. Arbabi , T. Ghoorchi and S. Hasani
  .
  S. Arbabi , T. Ghoorchi and S. Hasani
  In order to study the effect of delayed ensiling and application of some organic acid-based additives on fermentation of corn silage, chopped whole plant corn mixed with 4 different additives that consists of propionic acid in addition to control treatment without any additive. The additives were: (1) propionic acid (2) propionic acid (85%) + formic acid (15%) (3) calcium propionate and (4) propionic acid (80%) + formic acid (15%) + ammonia (5%). Ten gram per kilogram dry matter of each additives were mixed with chopped corn forage in 2 different times (0, 24 h). Silages were assessed and evaluated for appearance; DM and pH. CP, NDF, ADF, TVFA, WSC, aerobic stability and DM degradation each of treatments were determined after 60 days. Silages that was exposed to air for 24 h, before ensiling had better appearance quality in Filg’s method and whole additives had good effect on appearance quality compared with control group. In the method of DM and pH evaluation, all silages contained buffered propionic acid-based additive, were good and very good. These silages had lower pH than control ones (p<0.05). There was a significant interaction was between silages for concentration of dry matter (p<0.05). NDF in the silages (with propionic acid (85%) + Formic acid (15%)) was lower than the other silages while the contents of CP and TVFA increased (p<0.05) with addition of the additives especially additive containing propionic acid (85%) + formic acid (15%) (p<0.05). WSC in untreated silages was more than the other silage samples (p<0.05). Buffered propionic acid–based additives caused increase in the aerobic stability in treated silages compared with untreated silages. DM degradation (in situ) in untreated silages that delayed ensiled was lower other silages. Application of additives containing propionic acid (80%) + formic acid (15%) + ammonia (5%) resulted in highest DM degradation among experimental additives. Generally, treatment with propionic acid-based additives prevented the decrease of DM degradation (in vitro).
  S. Pashaei , M.A. Azari , S. Hasani , A. Khanahmadi and J. Rostamzadeh
  This study was carried out to investigate genetic diversity in Mazandaranian native cattle population comparised to the Holstein breed, using Inter Simple Sequence Repeats (ISSR) marker. A total of 175 animals, including 71 native and 104 cattle of Holstein breed were screened. The extraction of DNA samples were carried out, using modified salting out method. A 19-mer oligonucleotide, (GA)9C, was used as primer in PCR reactions. The PCR products showed 15 different fragments with length ranged from 120 to1600 bp in the two breeds.. Genetic variation indexes, including effective number of alleles, Shannon index, Nei’s gene diversity and standard genetic distance were estimated, using POPGene software. Generally, the estimated genetic variation indexes showed low levels of diversity in the two breeds. However, Nei's gene diversity and Shannon index estimation was observed almost two folds in native cattle compared to Holstein breed. Less levels of diversity in Holstein cattle may be because of applying intensive selection programs. Conversely, native cattle have been less affected by selection. Therefore, it seems that Mazandaranian native cattle probably are better for breeding programs than Holstein cattle. Results showed that ISSR Markers are reliable and can be used in genetic diversity investigations.
 
 
 
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