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Articles by S. Charkhkar
Total Records ( 2 ) for S. Charkhkar
  M. Soltanialvar , H. Shoushtari , M. Bozorgmehrifard , S. Charkhkar and F. Eshratabadi
  A total of 512 tissue samples collected from 30 farms located in various states of Iran during 2008-2009 as part of a program to monitor AIV infection in Iran poultry population. Avian influenza virus was isolated from poultry farms with history of respiratory illness and increased mortality. Nucleotide sequence analysis of five representative isolates confirmed that all isolates possessed one type of amino acid motif (R-S-S-R/GL) at cleavage site of HA, Deduced amino acid sequences showed the presence of L226 (234 in H9 numbering) in all five Iranian isolates which indicates a preference to binding of α (2-6) sialic acid receptors. The viruses' surface glycoproteins genes were >90% similar to those of A/Quail/Hong Kong/G1/97 (H9N2) lineage. The neuraminidase stalks regions in these viruses had no deletion as compared to that A/Dk/HK/ Y280/97 lineage (Ck/Bei-like viruses) and the 2 human isolates A/HK/1073/99, A/HK/1074/99. The hemadsorbing site of neuraminidase had up to 3 amino acid substitutions and is different from those of earlier Iranian viruses. Phylogenetic analysis HA and NA genes showed that they share a common ancestor Qa/HK/G1/97 isolate which had contributed internal genes of H5N1 virus.
  Y. Asadpour , M.H. Bozorgmehrifard , S.A. Pourbakhsh , M. Banani and S. Charkhkar
  The aims of the present study were to isolate and serotype, determine the Seroprevalence, Drug susceptibility and diagnosis of infection by Polymerase Chain Reaction (PCR). In this study 460 serum samples and 220 tracheal swabs, 90 ovaries and oviduct swabs, 90 misshapen egg shells swabs were collected from 22 broiler breeder flocks of 5 companies. Serological results showed that all of the 22 flocks (100%) were positive for ORT infection. Ornithobacterium rhinotracheale (ORT) antibodies were detected in 289 (62/83%) out of the 460 serum samples. ORT was detected from tracheal swabs of seven flocks (31/81% or 3/18% out of 220 tracheal swabs). There was significant correlation between flock different ages and ORT titers (p<0.05), but correlation of flock ages and ORT isolates was not significantly different (p>0.05). Seven flocks infected with ORT were detected positive in PCR but bacteria were Isolated from only five culture. No ovaries and oviducts, misshapen egg shell swabs yielded ORT. A 784 bp fragment of the 16S rRNA gene was amplified using ORT specific primers in the PCR. All the isolates were identified as serotype A by Rapid Agglutination Test. Drug sensitivity test using standard disk diffusion technique was performed with 27 antibiotics. Antibiotic susceptibility for Quinolons family was seen more than the others and Cephalosporins family except to Cephalexin. The isolates were 80-100% susceptible to Tetracycline family and the most antibiotic resistant were seen for Aminopenicillins, Polypeptides, Sulfanamides and 80-100% resistant to Aminoglycoside family. Eighty percent of the isolates were resistant to Licomycin and 60% were moderate sensitive to Lincomycin. This study is the first report of prevalence of ORT, bacterial isolation, biochemical characteristics, serotyping and molecular method (PCR) in broiler breeder flocks in Guilan province of Iran.
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