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Articles by S. Bhassu
Total Records ( 2 ) for S. Bhassu
  L.M. See , R. Hassan , S.G. Tan and S. Bhassu
  Random amplified polymorphic DNA was used to study the genetic diversity and assay polymorphisms among eleven populations of Macrobrachium rosenbergii in Malaysia. The RAPD procedure generated 205 markers in the 11 populations. and a large level of polymorphisms was observed in all populations. The unweighted pair group method with arithmetic averaging (UPGMA) clustering procedure based on the markers identified three major clusters among all populations. The results of the present study showed that RAPD based fingerprinting were a useful tool to assess the genetic variability of freshwater prawn, M. rosenbergii. Assessing high genetic variability within and among M. rosenbergii is considered important genetic resources as it has direct benefit for conserving wild stocks, greater potential for improvement and serves as invaluable resource for different selection criteria, especially when planning breeding or crossbreeding programs.
  N. Ghiasi , Z.A. Rashid , S. Hooshmand , K. Yusoff , S.G. Tan and S. Bhassu
  This study is to demonstrated that microsatellites markers developed for Tor tambroides can be used to amplify microsatellite loci in other family. It is assumed that microsatellite loci are more conserved for aquatic species compared to terrestrial ones due to aquatic environments are less mutagenic than terrestrial ones. Development of microsatellites still requires investment of time and resources. Thus using loci already developed in a related species may provide a cost-effective alternative to microsatellite isolation and development in a species of interest in present study, Probarbus jullieni. In this study we investigated the possibility of the conservation of microsatellite flanking regions among different species. Nine pairs of SSR primers, five gave very strong banding profile (SYK1, SYK 2, SYK 5 SYK 8 and SYK 9) which could be used for population studies by using the nested protocol. Results showed that SYK 2 and SYK 9 flanked the same (CA)n repeats and thus are highly conserved in a different species. The products of the SYK 5, 8 and 1 primer pairs showed differences in the microsatellite regions which they flanked in Probarbus jullieni when compared to those of the source species, Tor tambroides. The mean observed heterozygosity levels for all the primers ranged 0.23-0.81. The primers are all polymorphic with the mean number of alleles from 2-5.
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