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Articles by S. Banerjee
Total Records ( 4 ) for S. Banerjee
  A. Mujib , S. Banerjee and P.D. Ghosh
  Ornamentals are important groups of plants in plant kingdom. Conventionally, these groups of plants propagate vegetatively. During the last few decades plant tissue culture method has been used successfully for commercial production of a wide range of economic and elite plants including ornamentals. Although the in vitro technology assures true-to-type clones, tissue culture-induced variations are also common in plants. In this present review, the authors discussed various observed changes in tissue-culture regenerated plants. The changes have been categorized into three different types such as chimerical, temporary or physiological and somaclonal variation, both heritable and non-heritable in nature. The causes of variation, detection mode and related mechanism that contribute variation have been discussed in ornamentals. The communication also highlighted karyotypic alterations, sequence change, DNA methylation and the involvement of transposable elements with respect to cultural variation. The creation and rearrangement of new chimera, in vitro separation of chimera and identification of superior somaclonal variants may offer new varieties to growers for commercial plantations.
  S. Banerjee , G. Bandyopadhyaya , K. Chattopadhyay and B.D. Chattopadhyay
  Tobacco smoking is continuously increasing the risk of severe diseases like blood cancer, malfunctioning of blood immune system and peroxidative damage of erythrocytes, especially in developing countries. However, the effect of nicotine in the blood cells of protein malnourished condition and its amelioration by curcumin has not yet been studied. The present study is an attempt in that direction where female albino rats maintained under normal/protein restricted diets were subcutaneously injected with nicotine tartrate (2.5 mg kg-1 b.wt. daily for 21 days) and orally supplemented with curcumin (80 mg kg-1 b.wt.) and subsequently in vivo and in vitro experiments were performed. We report that nicotine alters the blood cell composition (RBC counts, haemoglobin content) and aggravates loss of DNA content and DNA damage in blood cells of protein malnourished female rats in comparison to normal diet fed group and curcumin significantly (p<0.001) attenuates nicotine-induced changes in blood cells of protein malnourished rats. In vitro study shows that curcumin strongly interacts with nicotine and also DNA and thereby protects the DNA from nicotine induced damages. This study also confirms that more free nicotine molecules are available in protein restricted condition which aggravates the nicotine induced toxic effect on blood cells. Thus protein malnourished animals are more susceptible to nicotine induced toxicity and curcumin is effective enough to prevent nicotine induced damage even in protein malnourished condition. The molecular mechanism underlying nicotine induced toxicity and prevention by curcumin is discussed in this study.
  S.K. Mondal , U.K. Mazumder , N.B. Mondal and S. Banerjee
  Microsomal stability of atenolol, propranolol HCl, verapamil HCl, imipramine HCl, midazolam HCl andrographolide and 14-Deoxyandrographolide was performed and quantitation was done by HPLC. The results with a single batch microsome showed good day-to-day reproducibility and variation in percent stability was within ± 5.71% of the average for all the compounds for the entire study period of 2 years. Microsome was found to be stable at -80 °C store for at least upto 2 years. Two batches of microsomes from each of 3 vendors were also used for stability study where similar type of animal, microsome preparation method and assay protocol were used. The findings demonstrated variations for both batch to batch and vendor to vendor were within 10%. Results indicated that variability in stability data can be minimized by selecting a fixed type of animal, keeping similar method of microsome preparation and following same assay protocol.
  S. Banerjee , A. Das , P. Chakraborty , K. Suthindhiran and M.A. Jayasri
  Araucaria cookie is an ornamental plant, which are evergreen conifer found in India and in many other European countries. Similarly Brassaia actinophylla is also an ornamental plant with its native from Java, Australia and in U.S. Though these plants are used for various purposes, the medicinal properties of the plants were not investigated. In our study, the two ornamental plants were chosen for screening both antioxidant and antimicrobial activity. The Leaves of the plants were used for preparing crude extract and was prepared by Soxhlet extraction method. For the extraction of the leave extracts, different solvents viz., methanol, chloroform and petroleum ether were used based on our preliminary data. The obtained extracts were condensed and stored. For the antioxidant and antimicrobial activity, the extractions were prepared into various concentrations. For the antioxidant activity DPPH was used as scavenger of the free radicals which showed the inhibition of percentage for Araucaria cookie was 63% and the inhibition percentage for Brassaia actinophylla 41%. For the antimicrobial activity the extracts were checked against two bacterial and two fungal pathogens. The phytochemical analysis assists in the study of the antioxidant and antimicrobial activity as to the probable compounds responsible for the activity. The result thus obtained provides a report of Brassaia actinophylla as a possible source of antioxidants and also the use of both extracts as a probable antimicrobial agent.
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