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Articles by S. Babu
Total Records ( 5 ) for S. Babu
  S. Babu , A. Sheeba , P. Yogameenakshi , J. Anbumalarmathi and P. Rangasamy
  In the present study, the salt tolerant genotypes were selected in both in vitro and in vivo condition. For in vivo condition all the genotypes were raised in three different salt affected areas having different soil and water pH and EC and observations were made on leaf proline content, Na+:K+ ratio, chlorophyll stability index and single plant yield. The following hybrids TS 6/TRY 1, TS 6/BTS 24, TS 29/CSR 27, TS 29/BTS 24, IR 58025 A/TRY 1, IR 58025 A/BTS 24 were stable for most of the traits in all the environments. Under in vitro condition, the response of plant cell to salt stress and the feasibility of selecting salt tolerant callus were studied. Callus was grown on agar solidified media containing 0.2, 0.4, 0.6, 0.8 and 1.0% NaCl salt. Parameters such as fresh weight of callus, callus morphology and proline content were studied. The callus growth decreased with increasing NaCl concentration in the medium. TS6/BTS 24, TS 6/Vytilla 1, TS 6/TRY 1, TS 29/BTS 24 and IR 58025 A/Vytilla 1 were the hybrids with high tolerance to salt stress in vitro.
  S. Babu
  An association analysis was carried out to obtain and interpret information on the nature of interrelationships between yield and yield related traits. Thirty four tea genotypes were used in this study to represent the high levels of correlation of four traits that were identified as important yield determinants such as dry weight of shoot, fresh weight of shoot, number of shoots/plant and leaf breadth. The inter correlation values were found to be highly significant, hence further partition the values by path analyses was carried out to have a more accurate selection of characters for improving the yield. Among the traits studied leaf breadth, dry weight of shoot and number of shoots/plant were found to have high positive direct effect on yield/plant. Based on both the correlation and path coefficient values, it is interesting to know that dry weight of shoot is a possible consideration for the direct selection of high yielding genotype in the early generation where the yield test is not being conducted.
  R. Nandakumar , S. Babu , G. Amutha , T. Raguchander and R. Samiyappan
  Phytotoxic metabolite produced by the five different isolates of Sarocladium oryzae, the rice sheath rot pathogen, was isolated from its in vitro culture filtrate to know the variability among isolates in terms of toxin production. The toxin was purified through solvent extraction and then by gel filtration on sephadex column. Carbohydrate and protein content of the toxin and the quantity of phytotoxin produced in planta were varied among isolates. Toxin from the five isolates also showed variation in its biological activity as detected in its ability to induce sheath rot symptoms on detached leaf sheath, greenhouse grown plants and to cause leakage of electrolytes from rice leaf sheath. The symptoms produced by the purified toxin were, symptoms similar to those of natural infection by the pathogen. The virulent isolates SO1 and SO2 produced more amount of toxin, more leakage of electrolytes and severe sheath rot symptoms. The in planta toxin production by different isolates was detected serologically, using the polyclonal antibody produced against SO1 toxin. Correlation between the variation in toxin production and the virulence of the pathogen was discussed.
  A. Nithya , K.M. Gothandam and S. Babu
  Outbreaks of illness due to human enteric pathogenic bacteria via fresh vegetables warrant intensive research on changing strategies of these bacteria in alterning their hosts for survival. The systemic infection of human pathogenic bacteria in plants and the plant growth stage at which they establish endophytic relationship is poorly understood. The issue is magnified in countries like India where the dietary habits are changing and consumption of fresh fruits and vegetables as salad has become a part in the everyday menu of most people. Most of the human pathogenic enteric bacteria are generally characterized by broad host ranges and these pathogens seem to exploit almost any change in human ecology that provides new opportunities for transmission. Because plants are not traditionally considered as hosts for human enteric pathogens, recent produce-associated outbreaks highlight important deficiencies in our understanding of the ecology of enteric pathogens outside of their human and animal hosts. This review focuses on understanding the human enteric pathogens that have developed abilities to colonize internal tissues of vegetables and fruits popularly consumed as salads, how and when do they enter plants and where do they localize in plant tissues. In addition, we have also highlighted the attempts made in detection and control of these bacteria in plant hosts. This understanding will help develop strategies towards vegetable food safety in a joint effort by agriculturalists, environmentalists, food processing agencies, whole salers and retailers, which will ultimately benefit every consumer.
  A. Nithya and S. Babu
  In this study, we optimized microbial killing methods specific to tomato, carrot and cucumber pulp were optimized by applying different treatments, moist heat, dry heat and UV-C irradiation. The ability of Listeria monocytogenes, Bacillus cereus, Escherichia coli, Shigella flexneri, Aeromonas hydrophila, Staphylococcus aureus and Salmonella enterica to grow on tomato, carrot, cucumber and onion pulp was investigated. Population of A. hydrophila and S. enterica were high in tomato, carrot and cucumber. The population of A. hydrophila was maximum at 36 h in tomato and carrot while it was high in cucumber at initial 24 h. The population of S. enterica was maximum at 24 h in tomato, 36 and 48 h in carrot and 48 h in cucumber. Onion pulp supported less growth of bacteria. The observed difference in the ability of different bacteria to grow in different vegetable pulp warrants development of vegetable specific sanitizing methods to avoid salad-borne enteropathogenic infection.
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