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Articles by S. Adeola
Total Records ( 2 ) for S. Adeola
  O.S. Folorunso , S. Adeola and A.O. Giwa Ajeniya
  Background and Objective: Medicinal plants are fast becoming essential pharmaceuticals for disease and infection management. The vast antimicrobial properties of these plants reside in the inhibitory properties of their endogenous secondary metabolites. Therefore, this study aimed to assess if the volatile oil of Syzygium samarangense inhibits enteric bacteria growth and its effect against the caseinolytic activity of the extracellular protease of Salmonella typhimurium. Materials and Methods: The volatile oil was extracted by hydrodistillation, while the antimicrobial assay was assessed with the microdilution method. The extracellular protease was partially purified by salting out, followed by size-exclusion chromatography. The mode of inhibition of this enzyme was deduced from the enzyme-substrate kinetics using a line-weaver burke plot. Results: The antimicrobial properties of the oil were reported against ten enteric bacteria. Proteus vulgaris has the highest IC50 value of 0.75±0.004% v/v, while S. typhimurium, the most sensitive bacterium, showed the lowest IC50 value of 0.17±0.005% v/v. The extracellular protease of S. typhimurium was partially purified to achieve 3.73 purification fold and 314.2 μmol min1 mg1 protein. The optimal caseinolytic activity of this enzyme was found at pH 7.5 and 40 °C. The protease showed significantly higher activity in the presence of Zn2+ (9.3±0.33 U min1) as compared to the control (7.0±0.58 U min1) (p<0.05), however, K+, Ca2+, Co2+, Ba2+, Pb2+ and Hg2+ considerably reduced the enzyme activity. The activity of this enzyme was competitively inhibited by the volatile oil as an inhibitor. Conclusion: The volatile oil of S. samarangense inhibited a wide range of enteric bacteria and, therefore proposed as a potential antimicrobial agent. Inhibiting the extracellular protease of S. typhimurium may be one of its modes of action against these pathogens.
  A.O. Boyo , M.B.O. Shitta , T. Oluwa and S. Adeola
  Dye sensitized solar cells, are one of the most promising devices for solar energy conversion into electricity, due to their reduced production cost and low environmental impact especially those sensitized by natural Dyes. This study used bitter leaf extract as the natural dyes for a dye sensitized solar cell (DSSC). The Soxhlet ethanol extraction method was used to extract the dye from the bitter leaf. The energy conversion efficiency and fill factor of this solar cells sensitized by bitter leaf extract is 0.69 and 0.81%. A phytochemical screening was performed for flavonoids and anthraquinones test. The extract obtained indicates the presence of flavonoids and less anthraquinones. We notice that the pH of extract solution has a significant effect on the performance of DSSCs. The efficiency was found to increase with decreasing pH and reached a maximum at the optimum pH 1.0. This might be due the absorption of more light by photoanode made from the bitter leaf extract at pH 1.0 and there is better DSSC stability as pH decreases. The results show a possible conversion of visible light into electricity using locally source conducting glass (mirror).
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