Asian Science Citation Index is committed to provide an authoritative, trusted and significant information by the coverage of the most important and influential journals to meet the needs of the global scientific community.  
ASCI Database
308-Lasani Town,
Sargodha Road,
Faisalabad, Pakistan
Fax: +92-41-8815544
Contact Via Web
Suggest a Journal
 
Articles by S. Zamanian
Total Records ( 2 ) for S. Zamanian
  S. Zamanian , G.H. Shahidi Bonjar and I. Saadoun
  This is the first report of antibacterial activity of Iranian actinomycetes isolates against Erwinia carotovora subsp. carotovora (Jones) Dye. E. carotovora subsp. carotovora is a soil born destructive plant pathogen with worldwide economical importance. Chemical measures have lost their attractiveness because of development of resistant strains of pathogens and due to undesirable effects on environment. Therefore, the studies on biological control of plant pathogens in soil have gained great importance worldwide. Actinomycetes, by virtue of their wide distribution, filamentous growth in soil, their ability to colonize the root surface and the rhizosphere, inhibitory effect on microorganisms and their ability to produce copious amounts of secondary metabolites especially antibiotics, role as a influential biological control agents have received special attentions. In order to obtain antibacterial antagonists from soil actinomycetes, a survey performed as follows. Soil samples taken from agricultural soils of Kerman. Serial dilutions provided and cultured in Casein Glycerin Agar (CGA). Over 110 isolates were screened among which one isolate showed high level of activity in Agar disk and Well diffusion methods against E. carotovora subsp. carotovora and identified as a new strain of Streptomyces plicatus (strain 101). This strain was propagated in submerged cultures and active crude was prepared upon which some biological characterization performed. High concentration of antibacterial agent was detected in 10th to 11th day in shake cultures. The active substance was water-soluble and insoluble in chloroform, hexan and dichloromethane. Minimal Inhibitory Concentration (MIC), by solving the crude extract in the dimethylsulfoxide plus methanol (v/v, 1:1) was determined as 5 mg mL-1. Longevity in vitro (LIV) of active crude of S. plicatus strain 101 against E. carotovora subsp. carotovora in soluble state determined about 30 days at room temperature. In thermal inactivation point studies, active crude retained activity up to 135°C. Antibacterial genes from this strain may be proper candidate for genetic engineering of plants for increased tolerance against the tested cosmopolitan bacterial pathogen.
  G.H. Shahidi Bonjar , S. Zamanian , S. Aghighi , P. Rashid Farrokhi , M.J. Mahdavi and I. Saadoun
  Ralstonia solanacearum has worldwide economical importance because of its destructive and soil-borne nature. Clearly chemical measures have lost their attractiveness because of development of resistant strains and undesirable effects on our environment. Consequently, biological control of pathogens is gaining great importance worldwide. To investigate for proper biocontrol agents and to obtain antibacterial antagonists from Iranian soil Actinomycetes, a vast survey was performed. Over 170 isolates of soil Actinomycetes were isolated and screened among which one isolate showed high level of activity in Agar disk and Well diffusion methods against R. solanacearum. It was identified as Streptomyces coralus strain 63. High concentration of antibacterial agent was detected at 8-11th day in shake cultures. Longevity in vitro of the active crude in soluble state determined about 40 days at room temperature. In thermal inactivation point studies, active crude retained activity up to 93°C. Antibacterial activity of the antagonists found in this study highlights their importance as candidates for further investigation in biological control of tested pathogenic bacteria.
 
 
 
Copyright   |   Desclaimer   |    Privacy Policy   |   Browsers   |   Accessibility