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Articles by S. Shabir
Total Records ( 2 ) for S. Shabir
  Phalisteen Sultan , A.S. Shawl , P.W. Ramteke , Arif Jan , Nahida Chisti , Neelofar Jabeen and S. Shabir
  A protocol has been developed for in vitro shoot proliferation from callus cultures of Podophyllum hexandrum Royle. Callus initiation occurs from root segments of established in vitro grown seedlings on Gamborg`s B5 media (half strength) supplemented with 2,4-D (0.5-1.5 mg L-1) and BA (0.2-1.0 mg L-1). The rhizome of this plant contains several important lignans and most important being podophyllotoxin, main precursor for anticancer drugs teniposide and ertoposide. In addition Podophyllum hexandrum has been reported to have radioprotection properties. Ruthless collection has led to the disappearance of this important medicinal plant from many areas of Himalayas. Thus there is need for immediate conservation of this important medicinal plant through tissue culture means. In the present study shoot proliferation occurs from callus cultures cultured on basal MS medium fortified with BA and IAA either alone or in combination (0.5-5.0 mg L-1) each. Regenerated shoots were rooted with high efficiency on MS medium fortified with activated charcoal (0.5-1.0%) and NAA (0.5-2.0 mg L-1). The rooted plantlets were transferred to green house in jiffy pots containing sand, soil and vermiculite in 1:1:1 ratio.
  A.S. Shawl , T. Kumar , Nahida Chishti and S. Shabir
  Essential oil of scented Geranium is widely used in high grade perfumery and cosmetic industries. It is also employed as a flavouring agent in many major food categories, alcoholic and soft drinks. Traditionally Geranium is used to staunch bleeding, healing of wounds, ulcers and skin disorders and also in the treatment of diarrohea, dysentry and colic. The oil has antibacterial and insecticidal properties and is profusely used in Aromatherapy. India imports 150t of geranium oil annually. Scented Geranium (Pelargonium sp.) was grown successfully in Kashmir. Experiments conducted at RRL Field Station Bonera Pulwama revealed that the morphological characters, growth behaviour, herbage and oil yield/ha were more or less similar to the hilly areas of South India. The aerial parts (Fresh flowers, stalks and leaves) on steam distillation yielded an essential oil 0.15% on fresh wt. basis. Fifty three chemical constituents representing >96% of the oil were identified by GC and GC-Mass analysis. Major constituents were citronellol (25.55%), geraniol (20.81%), citronellyl formate (8.04%) and isomenthone (7.59%). Linalool content was comparatively higher (11.94%) as compared to South Indian oil (6.7%). It is quite evident from the analysis that the oil produced under Kashmir conditions is quite competitive to best geranium oil produced in South India. Moreover the odour and evaluation studies by a leading perfumer has found that the oil is highly acceptable to the user industry.
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