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Articles by S. Moreno
Total Records ( 2 ) for S. Moreno
  E. A. C. Lima , M. L. Gomes , S. Moreno , M. T. Q. Marques , R. L. Jales and M. Bernardo-Filho
  The evidence that natural and synthetic drugs can affect radiolabeling or biodistribution of red cells in setting of nuclear medicine clinic has come to light only recently. A therapeutic drug can modify the nature/amount of technetium-99m (99mTc) radiopharmaceutical bound to blood elements and this may result in a unexpected behavior of the radiopharmaceutical. Once chayotte is used as food and also in folk medicine, we decided to evaluated the influence of this natural product on the labeling of blood elements with 99mTc and in the structural conformation of a plasmid pUC 9.1 trough gel electrophoresis analysis. Blood withdrawn from Wistar rats was incubated with the extract, (100% v/v).The blood samples were incubated with stannous chloride and with 99mTc. Plasma (P) and blood cells (BC) were isolated, also precipitated with trichloroacetic acid and soluble (SF) and insoluble fractions (IF) separated. A sample of blood was treated with ficoll-hypac, centrifuged and white cells were isolated. A solution of lymphocytes (2.5 mL) was obtained. After that, 0.2mL of a solution of lymphocytes was incubated with 0.1 mL of the vegetable extracts. A solution of stannous chloride and 99mTc were added. Lymphocytes and aqueous solution were separated. The %ATI bound to blood components was evaluated. The analysis of the results showed that there is not a decrease in %ATI in the blood constituents with chayotte extract. Although chayotte extract is not capable to alter the radiolabeling of. blood proteins and cells with 99mTc it was observed that the referred extract is capable to induce lesions in the plasmid DNA showing their possible oxidant properties. The studied natural product was not capable to oxide the stannous ions sufficiently to reduce the labeling of blood elements with 99mTc.
  G.F. Dire , E.A.C. Lima , M.L. Gomes , S. Moreno , M.V.C. Faria , R.L. Jales , M.T.J.A. Catanho and M. Bernardo Filho
  Natural products have been widely used by human beings. However, sometimes the biological effects of these products are not fully known. It is concerned that many natural medicines may contain potentially toxic ingredients and contaminants such as heavy metals. Red blood cells (RBC) and plasma proteins labeled with technetium-99m (99mTc) have several clinical applications and it has been reported that some natural products are capable of reducing the efficiency of this radio labeling. The aim of this work was to assess the oxidant or antioxidant action of the agents presents in the extract of Sechium edule verifying its effects on the labeling of blood elements with 99mTc and on the inhibition of the activity of acetyl cholinesterase (AchE). A freshly extract of Sechium edule was administered to Wistar rats during 15 and 60 days. After that, samples (0.5ml) of blood were incubated with stannous chloride (SnCl2) and 99mTc. The blood was centrifuged and plasma (P) and RBC were isolated. P and RBC were also precipitated with trichloroacetic acid and soluble (S) and insoluble (I) fraction (F) were determined. The results have shown that the referred extract was able to reduce the radio labeling in BC to 15 days of treatment (from 98.77%± 0.45 to 90.35%± 5.04) to 60 days of treatment (from 98.77%± 0.45 to 53.53%± 0.91), in IF-BC (from 90.36%± 2.34 to 21.20%± 2.36) and in IF-P to 15 days of treatment (77.20%± 2.19 to 53.26%± 6.69) to 60 days of treatment (77.20%± 2.19 to 11.01%±3.22). The analysis of toxicology was performed by the determination of acetyl cholinesterase activity. In the light of the results the referred extract has not shown inhibitory action due to the activity of the enzyme. In the light of the results obtained we suggest that the referred extract may induce the generation of activity metabolites with oxidant properties with direct action on the labeling process.
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