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Articles by S. Khalid
Total Records ( 3 ) for S. Khalid
  Yasmin, T. , S. Khalid , M..H. Soomro , S.A. Malik , H. Shah and I. Ahmad
  An attempt was made to investigate host-vector relationship of Banana Bunchy Top Disease. The affinity of different host plants was investigated via black banana aphid (Penlalonia nigronervosai for the transmission of BBTV to Canna (Canna indica), Cucumber (Cucumis sativus), Tobacco (Nicotiana tabacum cv. Samsun), Chenopodium (Chenopodium amranticotor) and Cotton (Gossypium hirsutum). The host plants, at 3-4 leaf stage were exposed to viruliferous P. nigronervosa for 24 hours. Similarly four different insect-vectors viz. Aphis gossypii, Myzus persicae, fiophalosiphum padj and BemisSa tsbaci, beside P. nigronervosa were tried for transmission studies under controlled conditions. The vectors were allowed inoculation access period of 24 hours following an acquisition-feeding period of 8-10 days in separate cages on healthy banana. None of the vectors except P. nigronervosa was found to transmit BBTV to banana. Likewise P. nigronervosa was also found restricted to its original host banana for propagation and BBTV transmission.
  Shah, H. , S. Khalid and I. Ahmad
  To ascertain the prevalence and distribution of four major pepper viruses viz. chili veinal mottle potyvirus (CVMV), cucumber mosaic cucmovirus (CMV), tobacco mosaic tobamovirus (TMV) & potato virus Y (PVY). Surveys of chili crop in three major provinces of Pakistan were conducted during 1996-98. At each location 3-5 farmer`s fields were inspected and samples showing virus-like symptoms were collected. A total of 500 samples (200 Punjab, 200 Sindh and 100 NWFP) were collected and tested through direct double antibody sandwich Enzyme-Linked Immunosorbent Assay (DAS-ELISA). CVMV & CMV appeared as the most prevalent viruses in almost all the surveyed areas. In Sindh, the incidence of CVMV, CMV, TMV, and PVY was 9, 16, 5 and 3.5%, respectively. In Punjab, CVMV appeared as the most important virus (63%) infecting pepper crop in all districts followed by CMV (20%). Co-infection of CVMV and CMV (10%) was recorded in Faisalabad, Multan and Darya Khan. In most CMV positive samples, TMV or CVMV or PVY were also detected (co-infection). In NWFP, CVMV incidence was 50% followed by CMV (17.8%). On the average, survey data showed that CVMV (40.6% incidence) is still the most prevalent virus infecting pepper followed by CMV (17.8%) in these three provinces. PVY was only detected in Sindh (3.5%) and NWFP (6%). TMV was not detected in any place of NWFP and Punjab, but detected in mixed infection in Sindh (5%). Thus there is 19.6% increase in CVMV incidence while frequency of other three viruses remained the same as compared to previous reports.
  Shah, H. and S. Khalid
  To identify source of resistance against the most important chili virus viz. chili veinal mottle virus (CVMV), eleven exotic chili lines (CV-1, CV-2, CV-3, CV-5, CV-6, CV-7, CV-8, CV-10, CV-11, CV-12, & CV-21) were screened in controlled conditions. The isolated virus was collected from Sindh province and maintained on Nicotiana tabacum cv. Samsun by successive mechanical inoculations confirmed by double antibody sandwich Enzyme-Linked Immunosorbent Assay (DAS-ELISA) at each step. The seedlings were raised in sterilized soil mixture of peat, clay and sand. Plants were mechanically inoculated (1:4 w/v) at 3-4 leaf stage and the new shoots were tested through DAS-ELISA two weeks post inoculation. The ELISA negative plants were decapitated and the new sprouting were re-inoculated and ELISA was performed two weeks after inoculation. Six lines (CV-1, CV-2, CV-3, CV-7, CV-11 & CV-12) showed no symptoms and were ELISA negative, while four lines (CV-5, CV-6, CV-10, & CV-21) displayed variable reaction resulting to 5, 13, 16 and 22% infection respectively while the susceptible check (CV-8) showed severe mottling with stunted growth (100 % infection) that gave high virus titer (>2). So, asymptomatic and ELISA negative lines showed
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