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Articles by S. K. Bhattacharya
Total Records ( 2 ) for S. K. Bhattacharya
  Sanjiva Bimal , Shubhankar K. Singh , P. K. Sinha , P. K. Das , H. Schallig , P. Das and S. K. Bhattacharya
  The efficacy of aqueous and freeze dried (FD) antigen was evaluated by direct agglutination test (DAT) for sero-diagnosis of visceral leishmaniasis. A total of 16 samples collected from 5 confirmed Kala-azar (KA) cases, 6 cases 2 each of filariasis, malaria, leprosy and 5 non-endemic healthy subjects were used in determination of cut off titre of both the antigens in DAT. A 1:800 for aqueous and 1:400 dilution for FD DAT antigen was found as cutoff values for differential diagnosis of KA cases from control subjects. Both antigens revealed 100% correlation in terms of sensitivity and specificity in diagnosis of KA cases and healthy subjects at these titres. Using both antigens and the cut off titres, sera from 26 individuals collected from different categories of leishmaniasis viz. Kala-azar pre-treatment 10, Kala-azar post-treatment 10, HIV-KA co-infection 1 and PKDL 5 were evaluated by DAT. Both the antigens showed equal sensitivity and specificity in diagnosis of different categories of leishmaniasis. The study concludes that though aqueous antigen showed equal sensitivity and specificity but FD antigen which is stable at 56oC do not require cold chain whereas aqueous antigen is unstable at room temperature and require proper cooling to retain its antigenic activity as studied in between day 1 and day 14, so the FD antigen should be used for routine diagnosis of KA cases particularly in field condition.
  Kai Man Kam , Cindy K. Y. Luey , Michele B. Parsons , Kara L. F. Cooper , G. B. Nair , M. Alam , M. Atiqul Islam , Danny T. L. Cheung , Y. W. Chu , T. Ramamurthy , G. P. Pazhani , S. K. Bhattacharya , H. Watanabe , J. Terajima , E. Arakawa , O.-A. Ratchtrachenchai , S. Huttayananont , Efrain M. Ribot , Peter Gerner-Smidt and Bala Swaminathan
  The pandemic spread of Vibrio parahaemolyticus is an international public health issue. Because of the outbreak potential of the organism, it is critical to establish an internationally recognized molecular subtyping protocol for V. parahaemolyticus that is both rapid and robust as a means to monitor its further spread and to guide control measures in combination with epidemiologic data. Here we describe the results of a multicenter, multicountry validation of a new PulseNet International standardized V. parahaemolyticus pulsed-field gel electrophoresis (PFGE) protocol. The results are from a composite analysis of 36 well-characterized V. parahaemolyticus isolates from six participating laboratories, and the isolates represent predominant serotypes and various genotypes isolated from different geographic regions and time periods. The discriminatory power is very high, as 34 out of 36 sporadic V. parahaemolyticus strains tested fell into 34 distinguishable PFGE groups when the data obtained with two restriction enzymes (SfiI and NotI) were combined. PFGE was further able to cluster members of known pandemic serogroups. The study also identified quality measures which may affect the performance of the protocol. Nonadherence to the recommended procedure may lead to high background in the PFGE gel patterns, partial digestion, and poor fragment resolution. When these quality measures were implemented, the PulseNet V. parahaemolyticus protocol was found to be both robust and reproducible among the collaborating laboratories.
 
 
 
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