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Articles by S. Chowdhury
Total Records ( 3 ) for S. Chowdhury
  M.N. Islam , M.N. Islam , S.U. Ahmed , M.M. Hossain and S. Chowdhury
  The effect of mulch and bulb size on the growth of onion was studied using four mulch treatments, viz., non-mulch i.e. control, ridge method of mulch, straw and black polyethylene mulch; and five different sizes of seed bulbs, viz., very small (0.5 g), small (1.0 g), medium (1.5 g), large (2.5 g) and very large (4.5 g). Mulching and bulb size had significant effect on plant height, leaf number, pseudostem diameter, root number and length. The interaction effect of mulch and bulb size was significant in plant height, root length and root number but it was non-significant in leaf number and pseudostem diameter.
  J. Ahmed , U.M. Seraj , M.A. Chowdhury and S. Chowdhury
  Molecular biology techniques were used for quick diagnosis of thalassemia in the present study. The incidence of thalassemia markers of both sexes, aged between 0.5-44 years, living in different areas of Chittagong, Bangladesh was studied. Information was collected from the subjects by questionnaire to find out the relationship of the thalassemia with sex, age, present illness, post illness, family relationships, personal history etc. Serum sample from the individuals were tested for routine hematological examination test and using molecular biology technique. Out of 45 subjects tested, a total of 29 cases (64.44%) were found to be thalassemia positive. Analysis of thalassemia markers in different sex groups showed that the incidence of thalassemia is greater in male (65.51%) than female (34.48%). The study also showed that thalassemia major were lower (34.48%) than the thalassemia minor (65.51%). Most of them are dependent on blood transfusion for their lives. It is concluded that the disease could be diagnosed quickly by electrophoresis and most important way to prevent the disease is to detect thalassemia carriers, parental diagnosis and pre-marriage counseling.
  U.M. Seraj , M.I. Hoq , M.N. Anwar and S. Chowdhury
  Attempts were made to isolate, purify and characterize antibacterial peptides from the hemolymph of the American cockroach Periplaneta americana. Both non-induced and induced hemolymphs were tested for their antibacterial activity against several Gram-positive and Gram-negative bacteria. Induction was done by injecting Escherichia coli into the abdominal cavity of the cockroach. A time-induction study showed that antibacterial peptides were induced as early as 1/2 an hour with a peak at 9 h which started to decline around 24 h. The non-induced hemolymph showed activity only against E. coli whereas induced hemolymph showed activity against several Gram-positive and Gram-negative bacteria as well as against one antibiotic resistant E. coli. The induced hemolymph was subjected to SDS-PAGE to estimate the number and molecular weight of proteins present in the crude hemolymph. Seven distinct protein bands were detected by SDS-PAGE. The hemolymph was then subjected to gel filtration chromatography to purify the proteins responsible for the antibacterial activity. Twenty fractions, one ml each, were collected and all the fractions were tested against those bacteria which previously showed sensitivity to the crude hemolymph. Only six fractions were found to be effective against the tested bacteria. The protein concentrations in the active fractions were determined by spectrophotometry. The active fractions were finally subjected to SDS-PAGE to determine the molecular weight of the protein(s) which were responsible for the antibacterial activity. There was one protein in all these six fractions except fraction F13 where an additional protein of 67kDa was present. The approximate molecular weight  of  the  isolated  antibacterial  protein  present  in  all the fractions was 61kDa. It was found that only 2.87 μg of the protein could inhibit bacterial growth whereas approx.10 μg of conventional antibiotics was required to obtain similar result.
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