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Articles by Rukhsana Bajwa
Total Records ( 7 ) for Rukhsana Bajwa
  Ghazala Nasim , Shahid Hameed Malik , Rukhsana Bajwa , M. Afzal and Salman Wajid Mian
  The sporocarps obtained from NARC, Islamabad and local market were utilized as a source of tissue culturing. Initial culturing was done on malt extract agar plates. The discs (0.5 cm in diameter) from actively growing culture plates of three different mushrooms were planted on the fresh media plates of Malt extract agar medium (MEA), Murashige and Skoog’s (MS) medium and potato dextrose agar (PDA) medium. Three replicates were taken in each case. An increase in diameter of the culture discs was recorded daily. The results indicted that the mycelial growth of Pleorotus ostreatus, varieties sajar, caju , citydeosus and Volvariella volvacea were maximum in medium plates containing MEA medium. The results were significant at P=0.05 level of significance. While potato dextrose agar medium (PDA), ended up with slowest growth.
  Rukhsana Bajwa , Nazma Aslam and Arshad Javaid
  The influence of three leguminous green manures viz. Trifolium alexandrianum, Medicago polymorpha and Melilotus parviflora on growth, yield and vesicular arbuscular mycorrhizal (VAM) colonization in maize (Zea mays L.) was examined and compared with recommended NPK fertilizers. The three green manuring plants contained 4.44, 2.45 and 3.17% nitrogen; 0.065, 0.089 and -0.122% phosphorus and 1.24, 1.89 and 0.8% potassium, respectively. NPK fertilizers suppressed VAM colonization whereas green manures generally favoured it. The study of correlation coefficient revealed that there was a variable pattern of correlation between N, P and K contents of green manures and various parameters of VAM colonization, at different growth stages. All the soil amendments enhanced shoot growth in terms of length and biomass, at all the three growth stages. Effect of Trifolium was similar to that of NPK fertilizers while Medicago and Melilotus were proved significantly superior than NPK fertilizers in increasing shoot biomass. Negative and significant correlation of shoot length and biomass with N contents of green manures was observed whereas with P content the correlation was positive and highly significant. Root length and biomass was increased by NPK fertilizers and either of the green manure amendment. Pattern of correlation of root length and biomass with N and P contents of green manures was similar to that of shoot. Highest cob biomass was recorded in mixed green manure followed by Medicago and Melilotus, respectively. Difference was significant as compared with control, NPK fertilizers and Trifolium. A highly significant and negative correlation was recorded between cob biomass and N content of the green manures. Pattern of correlation of root and shoot growth and cob biomass with various parameters of VAM colonization was variable and insignificant at different growth stages.
  Rukhsana Bajwa , Abida Yaqoob and Arshad Javaid
  Seasonal variation in vesicular arbuscular mycorrhizae (VAM) of eight aquatic and subaquatic vascular plant species viz.Thelypteris dentata,Marsilea minuta and Adiantum sp (pteridophytes), polygonum barbatumand Oenanthe javanica (dicotyledonous), Paspalum paspalodes, Saccharum sponitaneum and Vetiveria zizanioides( monocotyledonous) was studied. Pteridophytes in general showed maximum VAM colonization during spring with gradual reduction in the following seasons. VAM colonizaton in species of dicotyledonous and monocotyledonous showed a variable response to different seasons. A total of six mycorrhiza forming species belonging to a single genus, Glomus, were found to be associated with the test species. Among these G. fasciculatum and G. mosseae were the most common species and showed a marked variation and typical pattern of seasonal distribution i.e. maximum in spring with the gradual reduction in the following seasons. The other four species were rather inconsistent and lack any seasonal response.
  Rukhsana Bajwa , Naureen Akhtar and Arshad Javaid
  Effect of aqueous root and shoot extract of three allelopathic plants of family Asteraceae viz., Helianthus annuus L., Ageratum conyzoides L. and Cirsium arvense (L.) Scop., on growth of three pathogenic fungi namely Aspergillus niger Van Tieghem, A. fumigatus Fresenius and A. nidulans Eidam was studied in Hagem`s liquid nutrient medium. Root extract of H. annuus suppressed the growth of A. niger and A. fumigatus very effectively, A. conyzoides reduced the growth of A. fumigatus to a certain extent while the aqueous extracts of C. arvense induced lowest toxicity against the fungal species. A. nidulans exhibited complete resistance against all allelopathic extracts tested.
  Naureen Akhtar , Arshad Javaid and Rukhsana Bajwa
  Aqueous root, stem and leaf extracts of 0, 5, 10 and 15 % w/v of an allelopathic plant species viz. Cirsium arvense were evaluated for their potential to control germination and seedling growth of two weed species, Phalaris minor and Poa annua. All the aqueous extracts reduced germination in two test weeds. However, effect was insignificant statistically. Shoot length in P. minor was reduced significantly by 10 % stem extract while shoot length in P. annua remained unaffected by all the extracts. Root length was more susceptible to allelopathy and was reduced significantly in both the test weed species. In a similar experiment germination of P. minor and Medicago polymorpha was significantly reduced by 15 % leaf extract of Ageratum conyzoides. In both the test weeds, response of root and shoot length to aqueous extracts of A. conyzoides was similar to the response of test weeds to aqueous extracts of C. arvense in first experiment.
  Arshad Javaid , Rukhsana Bajwa and Iffat Siddiqi
  A field experiment was conducted to observe the VA mycorrhizal colonization and subsequent growth and yield of EM (Effective Microorganisms) treated and non-treated sunflower plants, at two growth stages viz., 40 and 70 days after sowing (DAS). In 40-day old plants, EM supported mycorrhizal association, which resulted in a parallel increase in number and biomass of leaves as well as stem length while stem biomass remained almost unaffected. Root growth was, however, slightly suppressed by EM application at this growth stage. EM failed to induce any remarkable change in extent of mycorrhizal infection 70 DAS. However, the arbuscular infection was enhanced by EM application that resulted in a parallel increase in vegetative and reproductive growth of the plant.
  Arshad Javaid , Rukhsana Bajwa , Nusrat Rabbani and Malika Uzma
  A variable response of crop growth, nodulation and VAM colonization to EM (effective microorganisms) application was observed when Vigna radiate (L) Wilczek was grown in farmyard manure (FYM) and Trifolium green manure (GM) amended soils with different histories of EM application. In soil 1 EM application was started six months prior to soil 2. Shoot growth was enhanced due to EM application in GM amended soil 1 but suppressed in soil 2 with either organic amendment. Root biomass was increased by EM application in FYM but reduced in GM amended soils, EM reduced pod dry biomass in soil 1 but enhanced in FYM amended soli 2. Irrespective of the soil type and organic amendment, nodulation was enhanced by EM application at vegetative growth stage. VAM colonization showed a positive response to EM application in both the soils. The response of crop growth was independent of the response of nodulation and VAM colonization to EM.
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