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Articles by Ruangyote Pilajun
Total Records ( 4 ) for Ruangyote Pilajun
  Anusorn Cherdthong , Metha Wanapat , Phongthorn Kongmun , Ruangyote Pilajun and Pichad Khejornsart
  Four rumen-fistulated, male swamp buffalo were randomly assigned according to a 4x4 Latin square design to evaluate the effect of the urea-treated rice straw to concentrate ratio (R:C) on rumen fermentation, nutrient digestibilities, microbial protein synthesis and cellulolytic bacterial population. Animals were fed R:C of 100:0, 75:25, 50:50 and 25:75, respectively. Results showed that digestibility of nutrients were significantly affected by R:C especially those of OM and fiber. However, digestibility of CP, ruminal NH3-N and plasma urea N were similar among treatments (p>0.05) whereas ruminal pH was decreased significantly (p<0.01) when concentrate ratio was increased. Total VFA concentrations and C3 were significantly different among treatments and were greatest at 50:50 of R:C supplementation (p<0.01). Total viable bacteria, proteolytic bacteria and bacteria cell count were not altered among treatments (p>0.05) whereas amylolytic bacteria, cellulolytic bacteria and fungal zoospore were significantly different (p<0.01), responding to a change in proportion of R:C. Moreover, using of real-time PCR technique provided that feeding of a 100% roughage remarkably increased these three cellulolytic bacteria numbers up to 3.54x109 copies mL-1 for F. succinogenes, 7.38x107 copies mL-1 for R. Flavefaciens and 5.80x106 copies mL-1 for R. albus in rumen digesta, respectively. It is most notable that F. succinogenes were the highest in population in the rumen of swamp buffalo. In addition, efficiency of rumen microbial N synthesis were enriched by R:C supplementation, especially at the ratio of 50:50 (p<0.05). Based on this study, it could be concluded that supplementation of R:C at 50:50 improved digestibilities of nutrients, ruminal ecology and microbial protein synthesis efficiency.
  Ruangyote Pilajun , Metha Wanapat , Chalong Wachirapakorn and Chainarong Navanukroaw
  Four, rumen-fistulated Holstein-Friesian steers were randomly assigned to four treatments according to a 4x4 Latin square design to study effects of coconut oil and sunflower oil ratio on rumen fermentation, rumen microorganisms and methane concentration in the rumen. The dietary treatments were ratios of coconut oil and sunflower oil at 100:0, 75:25, 50:50 and 25:75 for treatment 1-4, respectively. Steers were fed concentrate at 0.5% of BW (DM) and urea-treated rice straw was given ad libitum. The results were found that coconut oil and sunflower oil ratio did not affect feed intake and rumen microbial population except for total viable bacteria in which 75:25 ratio was the highest. Dietary treatments had affected nutrient digestibility and rumen fermentation especially 50:50 ratio. Methane concentration was linearly decreased when sunflower oil proportion increased. Nitrogen balance and microbial protein synthesis were similar among treatments, although microbial nitrogen supply tended to have a quadratic response to oil ratios. It is concluded that combined supplementation of coconut oil and sunflower oil could be beneficial to improve the rumen ecosystem and potential productivity in ruminants.
  Ruangyote Pilajun and Metha Wanapat
  An in vitro study was conducted to evaluate effect of coconut oil and Mangosteen Peel Powder (MPP) supplementation on methane production and methanogen population in rumen liquor of swamp buffalo. Completely randomized design was used with nine treatments including control and supplementation with coconut oil (3, 6% DM) and/or MPP (2, 4% DM). Supplementation of coconut oil and MPP significantly reduced methane production when compared with control group (p<0.05) however, increasing coconut oil and MPP levels decreased proportion of methane reduction. Population of methanogen was unchanged by supplementations (p>0.05) in contrast, the methanogen diversity slightly differed among treatments, especially when combinations with coconut oil and MPP used. The UPGMA dendrogram showed supplementation of coconut oil and MPP combination had pronounced effect on gel profile than that of single supplementation, especially combination of 6% of coconut oil with 2% of MPP which resulted in lowest band numbers. In addition, supplementation by high level of coconut oil or MPP did not show positive effects. Therefore, suitable level should not exceed than 6% for coconut oil and 4% DM for MPP supplementations, respectively.
  Ruangyote Pilajun and Metha Wanapat
  An in vitro study was conducted to evaluate effect of roughage to concentrate ratio (R:C) and coconut oil (CO) and wild almond seed oil (WO) supplementation on gas production, volatile fatty acid (VFA) concentration, methane production and dry matter disappearance. Completely randomized design was used for sixteen treatments. Treatments were 4 x 4 factorial arrangement where four of R:C ratios (100:0, 75:25, 50:50 and 25:75) and four of oil supplementation (un-supplement, 5% CO, 5% WO and 2.5% CO+2.5% WO). The potential extent of gas production was quadratically responded to R:C ratio (p<0.01) while gas production from all fractions of feed were suppressed by oil supplementation (p<0.05). Total VFA production, propionic acid proportion and calculated methane production were linearly increased with concentrate ratio; while oil supplementation decreased (p<0.05) these parameters especially when supplemented with wild almond seed oil. Dry matter disappearance at 24 h of incubation was increased with concentrate quantity but decreased by oil supplementation (p<0.05). It could be concluded that R:C ratio and seed oil remarkably influenced on fermentation end-product and gas production.
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