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Articles by Ria Millati
Total Records ( 4 ) for Ria Millati
  Rachma Wikandari , Ria Millati , Siti Syamsiyah , Ririn Muriana and Yuliana Ayuningsih
  Yeast isolate of Bekonang has been being applied for many years in ethanol home industry of Bekonang, Central Java, Indonesia. Its tolerance against furfural, Hydroxymethylfurfural (HMF) and acetic acid as the most by product components in acid hydrolysate was investigated. Isolate of Bekonang was cultivated on 50 g glucose L-1 at 30°C for 48 h under aerobic condition. Each flask was added with different concentrations of furfural (0.25, 0.5, 1, 1.5 g L-1), HMF (0.5, 1, 2, 3 g L-1) and acetic acid (0.75, 1.5, 3, 4.5, 6 g L-1). During fermentation, glucose consumption, ethanol yield and productivity were examined and compared with a reference. The results show that was completely consumed in the media with addition of furfural and HMF up to 0.5 g L-1 as well as in the presence of acetic acid up to 6 g L-1. Addition of 0.5 g L-1 of furfural and HMF only decreased both ethanol yield and productivity for <10%. However in the presence of furfural and HMF at 1 g L-1, the ethanol yields were decreased by 26 and 18.45%, respectively and ethanol productivities were decreased by 73 and 71%, respectively. Additions of acetic acid up to 1.5 g L-1 result in increasing both ethanol yield and productivity up to 6.96 and 6.89%, respectively. However, higher concentrations of acetic acid up to 1.5 g L-1 caused decreased ethanol yield and productivity. Addition of acetic acid in all tested concentrations results in acceptable range of ethanol yield and productivity compared with the reference. These findings show that furfural and HMF were inhibitors to the Isolate of Bekonang, while acetic acid can behave as a friend or foe depending on its concentration.
  Ria Millati , Rachma Wikandari , Elisabeth Titik Trihandayani , Muhammad Nur Cahyanto , Mohammad J. Taherzadeh and Claes Niklasson
  Oil Palm Empty Fruit Bunch (OPEFB) was hydrolyzed in a one-stage hydrolysis using dilute-sulfuric acid (0.2, 0.8%) at 170-230°C with a holding time of 5 and 15 min. The maximum yield of xylose was 135.94 g kg-1 OPEFB, obtained at 0.8% acid, 190°C and 5 min. The maximum yield of glucose was 62.70 g kg-1 OPEFB, obtained at 0.8% acid, 210°C and 5 min. Based on these results, two-stage hydrolysis was performed to produce hydrolyzates for the fermentation process. Hydrolyzate from the first stage was fermented by Mucor indicus while the hydrolyzate from the second stage was fermented by Saccharomyces cerevisiae. The corresponding ethanol yields were 0.45 and 0.46 g ethanol g-1 sugar consumed.
  Engkus Ainul Yakin , Zaenal Bachruddin , Ristianto Utomo and Ria Millati
  Objective: The study was conducted to determine the effect of manganese (Mn2+) addition on cocoa pod fermentation using Phanerochaete chrysosporium Pc2804. The enzyme activities of LiP and MnP, as well as the fiber fraction and digestibility of the cocoa pod, were evaluated. Methodology: The treatments were as follows: T1 = Cocoa pod fermentation without Mn2+, T2 = Cocoa pod fermentation with the addition of 200 μg Mn2+ g–1 substrate, T3 = Cocoa pod fermentation with the addition of 400 μg Mn2+ g–1 substrate, T4 = Cocoa pod fermentation with the addition of 600 μg Mn2+ g–1 substrate and T5 = Cocoa pod fermentation with the addition of 800 μg Mn2+ g–1 substrate. The observed variables were LiP and MnP activity, analysis of the fiber fraction including NDF, Acid Detergent Fiber (ADF), lignin, cellulose and hemicellulose and an analysis of in vitro digestibility. Results: The results showed that the addition of 600 μg Mn2+ g–1 substrate to cocoa pod fermentation with Phanerochaete chrysosporium produces the highest LiP and MnP activities: 0.199±0.00 and 0.098±0.00 U mL–1, respectively (p<0.05). The addition of 600 μg Mn2+ g–1 substrate to cocoa pod fermentation with Phanerochaete chrysosporium Pc2804 causes a decrease in NDF (75.83±0.78%), ADF (67.49±0.41%), lignin (25.27±0.41%), hemicellulose (8.34±1.05%) and cellulose (31.53±0.68%) (p<0.05). The addition of 600 μg Mn2+ g–1 substrate to cocoa pod fermentation with Phanerochaete chrysosporium Pc2804 produced the highest DM digestibility, which was 70.86±0.22% and the highest OM digestibility, which was 70.47±0.47% (p<0.05). Conclusion: The conclusion of this study is that the addition of 600 μg Mn2+ g–1 substrate to cocoa pod fermentation with Phanerochaete chrysosporium Pc2804 gives the best digestibility of the cocoa pod.
  Engkus Ainul Yakin , Zaenal Bachruddin , Ristianto Utomo and Ria Millati
  Background and Objective: This study aimed to determine the effect of cocoa pod fermented by Phanerochaete chrysosporium with the addition of 600 μg Mn2+ g1 substrate on the performance of the Javanese thin-tailed sheep. Materials and Methods: Twelve Javanese thin-tailed sheep with body weights of 15-17 kg were put into individual cages that were equipped with feed and drink containers. The study used a completely randomized design in a unidirectional pattern, with three treatments and four replications. The treatments involved the use of cocoa pod fermented by Phanerochaete chrysosporium with the addition 600 μg Mn2+ g1 substrate as thin-tailed sheep feed. The treatments were T1 = 40% concentrate+60% forage, T2 = 40% concentrate +42% forage +18% fermented cocoa pod and T3 = 40% concentrate +18% forage +42% fermented cocoa pod. Rations were prepared for a crude protein determination of 12%. Observed variables included dry matter intake, average daily gain (ADG) and feed conversion ratio. Results: The results showed that feeding Javanese thin-tailed sheep cocoa pod fermented by Phanerochaete chrysosporium with the addition of 600 Mn2+ g1 substrate produced the highest DM, OM and CP consumption with a composition of 40% concentrate +18% grass +42% fermented cocoa pod, at 1030.85±1.30, 672.26±0.89 and 123.80±0.99 g head1 day1, respectively (p<0.05). The highest DM, OM and CP in vivo digestibility was obtained with 40% concentrate +18% grass +42% fermented cocoa pod (67.69±0.50%, 66.33±0.54% and 60.05±0.66%, respectively). The highest average daily gain (145.46±1.05 g head1 day1) was obtained with 40% concentrate +18% grass +42% fermented cocoa pod. The lowest feed conversion ratio (7.39±0.08) was obtained with 40% concentrate +18% grass +42% fermented cocoa pod (p<0.05). Conclusion: It can be concluded that cocoa pod fermented by Phanerochaete chrysosporium with the addition of 600 μg Mn2+ g1 substrate can be used to substitute for up to 42% of dietary composition for Javanese thin-tailed sheep.
 
 
 
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