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Articles by Reza Pourrahim
Total Records ( 2 ) for Reza Pourrahim
  Shirin Farzadfar , Ali Ahoonmanesh , Gholam Hossein Mosahebi , Reza Pourrahim and Ali Reza Golnaraghi
  A survey was conducted to determine the occurrence and distribution of Cauliflower mosaic virus (CaMV) in several cruciferous crops in Iran. A total of 1451 symptomatic leaf samples were collected in 10 different Iranian provinces during the summers of 2004 and 2005 and then tested by enzyme-linked immunosorbent assay (ELISA) using specific CaMV-polyclonal antisera. Serological diagnosis was confirmed by herbaceous host range study, polymerase chain reaction (PCR) and sequencing. Spherical particles of approximately 50 nm in diameter were observed at electron microscope in symptomatic field samples. CaMV was detected in numerous cruciferous crops, with the highest incidence in cauliflower (Brassica oleracea var. botrytis) plantings. The virus occurred on B. oleracea var. acephala, B. oleracea var. capitata alba, B. oleracea var. italica, B. pekinensis, B. rapa, B. oleracea var. ganglyodes and Raphanus sativus in different provinces of Iran and in three brassica weeds (Rapistrum rugosum, Raphanus raphanistrum and Sisymbrium loeselii). Brevicoryne brassicae and Myzus persicae were the most widespread colonizing aphids of cruciferous crops in the surveyed areas, which also transmitted CaMV experimentally.
  Ali Reza Golnaraghi , Reza Pourrahim , Shirin Farzadfar , Kazusato Ohshima , Nooh Shahraeen and Ali Ahoonmanesh
  A total of 1,207 symptomatic and 2,677 randomly samples were collected from soybean fields throughout Iran during the growing seasons of 2004-2006 and tested by enzyme-linked immunosorbent assay (ELISA) using specific antibodies to Tomato yellow fruit ring virus (TYFRV), a newly proposed species in the genus Tospovirus. ELISA results were confirmed by electron microscopy, host range studies, serological analysis, polymerase chain reaction and limited to few affected plants, by sequencing of a 1200 bp DNA fragment corresponding to the N gene of TYFRV. The virus was largely detected in the main soybean growing provinces of Iran with an overall incidence of 6.1%, even if its introduction in Iranian soybean fields seems to be only recent. In this study, Thrips tabaci was identified for the first time as a vector of the virus, but no transmission by soybean seeds was evidenced for the same virus. TYFRV was also detected in some weed species and for the first time in Datura metel and D. stramonium, as confirmed by biological and molecular assays.
 
 
 
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