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Articles by Reza Mohammadi
Total Records ( 3 ) for Reza Mohammadi
  Reza Mohammadi , Mohammad Armion , Akbar Shabani and Amir Daryaei
  This study was undertaken to determine the yield performance of 15 improved durum wheat genotypes selected from Iran/ICARDA joint project in 12 environments during 2004-06 in Iran. Our results showed that contributions to treatment main-effects were: genotype (2.85%), environment (61.59%) and GE (14.86%). Analysis of the interaction revealed decreasing magnitude of contributions to the GE sum of squares in the order: Interaction Principal Component Axis 1 (IPCA1) = 28.2%, IPCA2 = 25.04%, IPCA3 = 18.98% and IPCA4 = 8.52%. In this study the IPCA scores presented a disproportionate genotype response, which was the major source of variation for crossover GE interaction. GE interaction patterns revealed by AMMI biplot analysis indicated that the durum wheat genotypes are narrowly adapted. No genotype has superior performance in all environments. The genotype Mrb3/Mna-1 was the best at combining yield stability and productivity. The genotypes 12A-Mar8081 and 14A-Mar8081 had the most stability but low yielding. Mna-1/Rfm-7 had the highest yield performance and relative widely adapted to across environments.
  Sayyed Saeid Pourdad and Reza Mohammadi
  In this study, some phenotypic stability parameters; ecovalence (W2i), regression coefficient (bi), coefficient of determination (R2i), coefficient of variation (CVi), stability variance (S2i) AMMI stability value (ASVi) and TOP (proportion of environments in which a genotype ranked in the top third), were used to select among 17 spring safflower genotypes for yield performance and stability simultaneously across 26 environments under rain-fed conditions of Iran during growing seasons 2004-06. The results of AMMI analysis showed that 83.78% of the total sum squares (SS) was attributable to environment effects, only 1.37 and 14.85% to genotype and GE interaction effects, respectively. The results showed none of the parametric statistics per se was useful for selecting high yielding and stable genotypes. By simultaneous selection for yield and stability the genotypes G9, G10 and G11 were the best whereas the G1 and G17 with the highest yield performance were the most instable. In conclusion, both of yield and stability should be considered simultaneously to exploit the useful effect of GE interaction and to make selection of the genotypes more precise and refined.
  Alireza Saeedinia , Mehdi Shamsara , Ali Bahrami , Mehdi Zeinoddini , Mohammad Ali Naseeri-Khalili , Reza Mohammadi , Narges Malek Sabet and Hosseinali Sami
  Human¬† granulocyte-colony¬† stimulating¬† factor (hG-CSF) was expression in a eukaryotic system, Pichia pastoris. For this, hG-CSF cDNA was amplified using reverse transcription-polymerase chain reaction (RT-PCR) and inserted into pPIC9 vector. The recombinant plasmid was electroporated into the P. pastoris. The recombinant hG-CSF was successfully expressed by in this system. The protein of approximately 18 kDa was secreted into the culture supernatant when induced with methanol. Western blot showed that the recombinant hG-CSF expressed in P. pastoris had a high specificity to monoclonal antibody against hG-CSF. The expression of hG-CSF was determined 3.1 mg L-1 in shake flask culture by ELISA method.
 
 
 
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